The pharmacokinetics of levofloxacin mesylate in healthy adult giant panda is unknown. In this study, the pharmacokinetics of levofloxacin after intramuscular administration at a dose of 2 mg/kg and oral administration at a dose of 3 mg/kg in healthy adult giant pandas was determined. Levofloxacin concentrations in plasma were determined using liquid chromatography. In the levofloxacin intramuscular administration group, the absorption and elimination half-lives of the drug were determined to be 0.123 (range: 0.02) hr and 5.402 (range: 0.70) hr, respectively. In the levofloxacin oral administration group, the absorption and elimination half-lives were determined to be 0.325 (range: 0.02) hr and 7.143 (range: 0.63) hr, respectively. Furthermore, the blood–drug concentration of levofloxacin was found to be above 1 μg/ml after 8 hr of intramuscular administration and above 0.5 μg/ml after 12 hr of oral administration. In this study, HPLC conditions and pretreatment methods of plasma samples were optimized and a detection method was established. Our results indicated that in healthy adult giant pandas, levofloxacin was rapidly absorbed and slowly eliminated. This study will therefore provide to be a guide in veterinary research and will be helpful in the rational use of levofloxacin in giant panda.

中文翻译：

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不同途径单剂量给药后甲磺酸左氧氟沙星在健康成年大熊猫体内的药代动力学

甲磺酸左氧氟沙星在健康成年大熊猫体内的药代动力学未知。本研究测定了健康成年大熊猫肌肉注射2mg/kg剂量和口服3mg/kg剂量后左氧氟沙星的药代动力学。使用液相色谱法测定血浆中的左氧氟沙星浓度。在左氧氟沙星肌肉注射组中，药物的吸收和消除半衰期分别测定为 0.123（范围：0.02）小时和 5.402（范围：0.70）小时。在左氧氟沙星口服给药组中，吸收和消除半衰期分别测定为 0.325（范围：0.02）小时和 7.143（范围：0.63）小时。此外，肌肉注射 8 小时后左氧氟沙星的血药浓度高于 1 μg/ml，口服 12 小时后高于 0.5 μg/ml。本研究对血浆样品的HPLC条件和前处理方法进行了优化，建立了检测方法。我们的研究结果表明，在健康的成年大熊猫中，左氧氟沙星被迅速吸收并缓慢消除。因此，本研究将为兽医研究提供指导，有助于大熊猫合理使用左氧氟沙星。左氧氟沙星吸收迅速，消除缓慢。因此，本研究将为兽医研究提供指导，有助于大熊猫合理使用左氧氟沙星。左氧氟沙星吸收迅速，消除缓慢。因此，本研究将为兽医研究提供指导，有助于大熊猫合理使用左氧氟沙星。