Identification of optimal and novel reference genes for quantitative real-time polymerase chain reaction analysis in grapevine,Australian Journal of Grape and Wine Research

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Identification of optimal and novel reference genes for quantitative real-time polymerase chain reaction analysis in grapevine
Australian Journal of Grape and Wine Research ( IF 2.5 ) Pub Date : 2021-02-09 , DOI: 10.1111/ajgw.12483
T.‐L. Wei _{1,

2} , H. Wang _{1,

2,

3} , M.‐S. Pei _{1,

2} , H.‐N. Liu _{1,

2} , Y.‐H. Yu _{1,

2} , J.‐F. Jiang ₃ , D.‐L. Guo _{1,

2}

Affiliation

Quantitative real-time polymerase chain reaction (qRT-PCR) has the advantages of sensitivity, specificity and repeatability, and is commonly used to investigate the expression levels of genes. The selection of appropriate endogenous reference genes is the pre-condition for acquiring the desired results of qRT-PCR. This study aimed to identify and screen the most suitable reference genes for grapevines.

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