As an indispensable chemical raw material, tannins are widely used in industrial production. The crude tannins extracted from plants are difficult to be used directly because of their low purity. Therefore, tannins purification are particularly important. In this study, an economical and efficient method was developed for the purification of novel vegetable tannin resources from Coriaria nepalensis bark by macroporous resin. The purity of the obtained tannin was increased significantly from 32.5%–70.6% by optimal adsorption and desorption process conditions with D101 macroporous resin. The isothermal adsorption results illustrated by Langmuir and Freundlich equations showed that the adsorption of tannin with macroporous resin was in accordance with the exothermic process. Furthermore, the structure of the purified tannins was investigated by MALDI-TOF MS. The results indicate that the main structural compositions of the obtained tannins were Tri-O-galloylglucose, Tetra-O-galloylglucose, Penta-O-galloylglucose and Hexa-O-galloylglucose. The hydroxyl and DPPH radical scavenging rates of the purified tannins at a concentration of 256 μg mL⁻¹ increased to 86.33% and 89.08%, respectively. When the concentration was 512 μg mL⁻¹, the reducing ability of ferricyanide to the ferrous form reached to 0.88. Moreover, the minimal inhibitory concentrations for Staphylococcus aureus and Escherichia coli were 32 μg mL⁻¹, which is superior to commercial tannic acid. In addition, further application experiment results show that Coriaria nepalensis bark tannins can be effectively hydrolyzed under the action of tannase to obtain a high yield of gallic acid (53.2%).

单宁作为必不可少的化学原料，已广泛用于工业生产中。从植物中提取的粗制丹宁酸纯度低，难以直接使用。因此，单宁的纯化特别重要。在这项研究中，开发了一种经济有效的方法来纯化来自尼泊尔Cor的新型植物单宁资源树皮由大孔树脂制成。通过最佳的D101大孔树脂吸附和解吸工艺条件，获得的单宁酸纯度从32.5％–70.6％显着提高。用Langmuir和Freundlich方程表示的等温吸附结果表明，单宁与大孔树脂的吸附符合放热过程。此外，通过MALDI-TOF MS研究了纯化的单宁的结构。结果表明，所得单宁的主要结构组成为三-O-半乳糖基葡萄糖，四-O-半乳糖基葡萄糖，五-O-半乳糖基葡萄糖和六-O。-半乳糖基葡萄糖。浓度为256μgmL ^-1的纯化单宁的羟基和DPPH自由基清除率分别增加至86.33％和89.08％。当浓度为512μgmL ^-1时，铁氰化物还原为亚铁形式的能力达到0.88。此外，最小抑制浓度为金黄色葡萄球菌和大肠杆菌为32微克毫升^-1，这是优于商业单宁酸。另外，进一步的应用实验结果表明，在鞣酸酶的作用下，可有效地水解马i树皮单宁酸，从而获得高产率的没食子酸（53.2％）。