The present investigation of new hydrogel based on chitosan was prepared by radical graft copolymerization of N-vinylpyrrolidone and 4-vinylpyridine with chitosan and cross-linking by N, N′-methylen-bis-acrylamide. The swelling behavior of the prepared hydrogel, with the optimal amount of gelator, was investigated. The hydrophilicity, and pH sensitivity of gel was suitable for trypsin carry. Trypsin was loaded onto the hydrogel enternal pores and surfaces by electrostatic interaction and hydrogen bond attachment. Several parameters, such as adsorbtion of pH value, loading time, and buffer solution concentration on trypsin loading amount, also enzyme activity were examined. According to the kinetic and FTIR, SEM spectroscopy analysis data trypsin was combination by the hydrogen bond and electrostatic interaction to the chitosan based structure. The hydrogen bonds were formed between -NH₂, -CH₂OH, >C=O, pyridine ring, -OH, and -NH- groups of carrier with -NH₂, -COOH, -OH, >C=O, -NH-, disulfide bridge, ester groups of trypsine. In additional, at pH=5–8, the functional groups in the gel are partially ionized and take the form of -NH₃⁺, -OH₂⁺, >COH⁺. The protein is the same degree ionized in that medium, and the protein interacts electrostatically with the carrier.

本研究基于壳聚糖的新型水凝胶是通过N-乙烯基吡咯烷酮和4-乙烯基吡啶与壳聚糖的自由基接枝共聚反应，并通过N，N'-亚甲基双-丙烯酰胺。研究了在最适量的胶凝剂条件下制备的水凝胶的溶胀行为。凝胶的亲水性和pH敏感性适合于胰蛋白酶的携带。胰蛋白酶通过静电相互作用和氢键连接被加载到水凝胶肠孔和表面上。考察了pH值的吸附，上样时间，缓冲液浓度对胰蛋白酶上样量的影响以及酶的活性。根据动力学和FTIR，SEM光谱分析数据将胰蛋白酶通过氢键和静电相互作用结合到壳聚糖基结构上。带有-NH ₂的载体的-NH ₂，-CH ₂ OH，> C = O，吡啶环，-OH和-NH-基团之间形成氢键，-COOH，-OH，> C = O，-NH-，二硫键，胰蛋白酶的酯基。另外，在pH = 5-8时，凝胶中的官能团部分被离子化，呈-NH ₃ ⁺，-OH ₂ ⁺，> COH ^+的形式。蛋白质在该介质中的离子化程度相同，并且蛋白质与载体发生静电相互作用。