The resistance of hospital bacterial isolates against traditional germicides, which used frequently, is one of the important factors contributing to emerging nosocomial infections. Moreover, due to having the side effects of chemical substances, the development of novel low-risk natural compounds seems necessary for control the spread of resistant pathogens in hospital environments. The aim of this study was to compare the effect of carvacrol and glutaraldehyde against two common hospital acquired pathogens, including Pseudomonas aeruginosa and Staphylococcus aureus. In this study 365 samples were collected from different wards of hospitals of Khorramabad, Iran. One hundred and sixty samples were identified as P. aeruginosa and S. aureus by using standard microbiological methods. Then the antibacterial effects of four combinations including carvacrol+ethanol, carvacrol+dimethyl sulfoxide (DMSO), glutaraldehyde 2%, and pure glutaraldehyde (50%) were evaluated and determined using dilution broth and disk diffusion methods. Our results showed that the carvacrol had more antibacterial effects against selected bacteria compared to glutaraldehyde. Moreover, the optimal time and concentration of carvacrol+ethanol against hospital isolates of P. aeruginosa and S. aureus was determined after 1 h at concentration of 64 μl/ml and 8 μl/ml, respectively. In conclusion by comparing the results of carvacrol and glutaraldehyde, seem that carvacrol, as an herbal and natural agent, may be a suitable alternative to glutaraldehyde in hospital equipment’s’ sterilization.

医院细菌分离物对常用的传统杀菌剂的耐药性是导致新发医院感染的重要因素之一。此外，由于具有化学物质的副作用，新的低风险天然化合物的开发似乎对于控制耐药病原体在医院环境中的传播是必要的。本研究的目的是比较香芹酚和戊二醛对两种常见的医院获得性病原体（包括铜绿假单胞菌和金黄色葡萄球菌）的作用。在这项研究中，从伊朗霍拉马巴德医院的不同病房收集了 365 个样本。一百六十个样本被鉴定为铜绿假单胞菌和金黄色葡萄球菌通过使用标准的微生物方法。然后使用稀释肉汤和圆盘扩散法评估和确定四种组合的抗菌效果，包括香芹酚+乙醇、香芹酚+二甲基亚砜 (DMSO)、2% 戊二醛和纯戊二醛 (50%)。我们的研究结果表明，与戊二醛相比，香芹酚对选定的细菌具有更强的抗菌作用。此外，香芹酚+乙醇对医院铜绿假单胞菌和金黄色葡萄球菌分离株的最佳时间和浓度1 小时后分别以 64 μl/ml 和 8 μl/ml 的浓度测定。综上所述，通过比较香芹酚和戊二醛的结果，香芹酚作为一种草本植物和天然药剂，似乎可以替代戊二醛用于医院设备的消毒。