Pyrus communis is a difficult-to-root species, and its optimal rooting depends on applying an accurate concentration of various chemicals. Since statistical modeling can be applied to estimate the approximate range of factors needed for the success of in vitro plant propagation, we tried to optimize the rooting medium using response surface methodology. Therefore, sucrose and polyvinyl alcohol were selected as independent variables in the central composite design of response surface methodology with α = 1. Based on the pre-test, it was found that the independent variables for the root formation were 2 to 3% sucrose and 0.5 to 1.5 g L⁻¹ polyvinyl alcohol in studied cultivars. The statistical analysis results showed that the quadratic models were significant, with high R² values > 0.9 for rooting rate response. The optimized values for independent variables in rooting of the studied cultivars included 2% sucrose, 1.5 g L⁻¹ polyvinyl alcohol in ‘Williams’; 2.85% sucrose, 1.47 g L⁻¹ polyvinyl alcohol in ‘Natanzi’; and 3% sucrose, 0.99 g L⁻¹ polyvinyl alcohol in ‘Ghosi’. A separate validation experiment detected that the experimental values of the studied responses are in close agreement with predicted values in all cultivars. For the first time, the methodology was developed using a central composite design to optimize the rooting in P. communis.

梨属（Pyrus communis）是难以生根的物种，其最佳生根取决于对各种化学药品的精确浓度。由于统计模型可用于估算成功实现体外植物繁殖所需因素的大致范围，因此我们尝试使用响应面方法优化生根培养基。因此，在α  = 1的响应面方法的中心复合设计中，选择蔗糖和聚乙烯醇作为自变量。根据预测试，发现根形成的自变量为2％至3％蔗糖， 0.5至1.5 g L ^-1研究品种中的聚乙烯醇。统计分析结果表明，二次模型很明显，生根速率响应的R ²值> 0.9较高。研究品种生根的独立变量的最佳值包括2％蔗糖，1.5 g L ^-1聚乙烯醇（在“威廉姆斯”中）；2.85％的蔗糖，'Natanzi'中的1.47 g L ^-1聚乙烯醇; 在“ Ghosi”中添加3％蔗糖和0.99 g L ^-1聚乙烯醇。单独的验证实验检测到，所研究响应的实验值与所有品种的预测值均非常接近。该方法首次使用中央复合设计开发，以优化植根芦苇。