A total of 33,206 wild boars (Sus scrofa) hunted throughout the province of Girona (northeastern Spain) were submitted to a game processing plant during four hunting seasons (2014–2015 to 2017–2018). This province is divided into seven districts with different habitat types for wild boar populations. Muscle samples from each wild boar were tested for Trichinella spp. infection by artificial digestion following Regulations 2075/2005 and 2015/1375 of the European Union. Larvae from positive muscle samples were characterized at the species level in the National Reference Laboratory for Trichinella in Spain using a multiplex PCR analysis. Trichinella larvae were found in a total 112 wild boars (0.34%; 95% IC 0.27–0.39%). Results indicated an annual ascending trend in the percentage of infected wild boars; however, this trend was small, as shown by the low values of both Kendall’s tau (0.328; p=0.012) and the linear fit slope (b=8.864E-5). The prevalence of Trichinella spp. per district and hunting season was positively correlated with hunting yield (Spearman’s rank correlation r_s = 0.440; p = 0.024). Spring prevalence (0%) was significantly lower than in other seasons (0.29–0.33%), although this effect could be explained by the scarcity of captures during the months of April, May, and June. The finding of one positive animal weighing only 7.5 kg indicates that piglets can be infected by Trichinella at an early age, but a significantly higher proportion of positives was found among adult wild boars exceeding 50 kg. The molecular identification of 112 Trichinella isolates revealed the predominance of Trichinella spiralis (n = 109) over Trichinella britovi (n = 3). These findings indicate that Trichinella infection in wild boar remains a public health problem in northeastern Spain.

在四个狩猎季节（2014-2015年至2017-2018年），在赫罗纳省（西班牙东北部）全省猎获的总共33,206头野猪（Sus scrofa）被送往游戏加工厂。该省分为七个地区，分别为野猪种群提供不同的栖息地类型。测试来自每个野猪的肌肉样品的旋毛虫属物种。根据欧盟法规2075/2005和2015/1375进行的人工消化感染。在西班牙的旋毛虫国家参考实验室中，使用多重PCR分析技术对来自阳性肌肉样品的幼虫进行了物种级别的鉴定。旋毛虫在总共112头野猪中发现了幼虫（0.34％； 95％IC 0.27–0.39％）。结果表明，受感染野猪的百分比呈逐年上升趋势。然而，这一趋势很小，如肯德尔tau（0.328; p = 0.012）和线性拟合斜率（b = 8.864E-5）都较低。旋毛虫属的流行。每区，狩猎季节呈正狩猎产量相关（Spearman秩相关ř_小号= 0.440; p= 0.024）。春季患病率（0％）明显低于其他季节（0.29–0.33％），尽管这种影响可以用四月，五月和六月的捕获量不足来解释。发现一只体重仅7.5千克的阳性动物表明，仔猪可在早期就被旋毛虫感染，但在体重超过50千克的成年野猪中发现阳性的比例要高得多。对112种旋毛虫的分子鉴定表明，旋毛虫（n = 109）优于小旋毛虫（n = 3）。这些发现表明旋毛虫 在西班牙东北部，野猪感染仍然是一个公共卫生问题。