Neofusicoccum parvum is a recently reported pathogen affecting Theobroma cacao L., and has been isolated from symptomatic pods on Oahu and Hawaii Islands. Determining infection routes and virulence are essential for assessing the impact of N. parvum on cacao production and developing effective disease management strategies. Infection routes were determined by inoculating unwounded stems and pods with six isolates of N. parvum alongside Lasiodiplodia theobromae and Phytophthora palmivora. Fifty percent of unwounded stems inoculated with P. palmivora developed lesions, but only a single lesion developed following inoculation with N. parvum (isolate H44). L. theobromae and the remaining N. parvum isolates did not induce lesion development on unwounded stems. In contrast, all N. parvum and L. theobromae isolates produced lesions on 40–100% of unwounded pods of GNV 164 and GNV 360. Low incidences of infection were observed in unwounded pods of ICS 95 (0–66.7%), SHRS 21 (0–75%), and SHRS 33 (0–20%). On wounded pods, all pathogen species produced similar size lesions, ranging from 1.90 to 7.60 cm four days after inoculation. Results from this study show that all three species can produce high rates of pod infection on some clones in the absence of wounds, but stem infection is less likely. In addition, this is the first report of L. theobromae infecting cacao pods and P. palmivora infecting stems in the absence of wounds.

新镰刀菌是最近报道的影响可可树的病原体，已经从瓦胡岛和夏威夷群岛的有症状豆荚中分离出来。确定感染途径和毒力对于评估细小猪笼草对可可生产的影响和制定有效的疾病管理策略至关重要。感染途径是通过接种未受伤的茎与六株荚确定N.孢子虫一起Lasiodiplodia theobromae和疫palmivora。未受伤的百分之五十茎接种P. palmivora开发病变，但仅单个病灶开发以下接种N.孢子虫（隔离H44）。L. theobromae和其余的小孢杆菌分离株未诱导未伤口茎上的病灶发展。相反，所有N.孢子虫和L. theobromae菌株产生的病变在ICS 95（0-66.7％），SHRS 21的未受伤的豆荚，观察360感染的发病率低164 GNV和GNV的未受伤的豆荚40-100％ （0–75％）和SHRS 33（0–20％）。在受伤的豆荚上，接种后四天，所有病原体种类都产生相似大小的病变，范围从1.90至7.60 cm。这项研究的结果表明，在没有伤口的情况下，这三个物种均可在某些克隆上产生较高的荚果感染率，但茎感染的可能性较小。此外，这是L. theobromae的首次报道在没有伤口的情况下感染可可豆荚和棕榈果蝇感染茎。