By feeding on grapevine roots, the ectoparasite Xiphinema index transmits grapevine fanleaf virus (GFLV), causing fanleaf degeneration in the plant. Therefore, rootstock breeding programs are considered a key to resistance to both threats. Previous screening depends on long-lasting inoculation experiments and mainly focuses on evaluating gall formation on the parasitized roots to identify potential resistance in grapevines. Since the roots of grapevine rootstocks resistant to X. index and even those of susceptible cultivars showed no reliable gall formation after parasitism, and further, root thickening was regularly found on non-inoculated control plants, nematode reproduction rates were used to validate rootstock phenotypes regarding susceptibility or resistance to X. index. For our approach, a glass vial assay was performed, and 35 days after inoculation the nematode population was determined by counting juveniles after the extraction. Reproduction rates were high on susceptible rootstock cultivars and decreased the more resistant a cultivar is considered. Besides focusing solely on root gall formation, the nematode reproduction rate should be included as a reliable indicator for resistance screening. The described assay setup enables the evaluation of resistance levels by fast screening to select promising rootstock candidates.

通过以葡萄的根为食，外寄生物Xiphinema指数会传播葡萄的扇叶病毒（GFLV），从而导致植物中的扇叶退化。因此，砧木育种计划被认为是抵抗两种威胁的关键。先前的筛选取决于长期的接种实验，并且主要侧重于评估被寄生的根上的胆汁形成，以鉴定葡萄树的潜在抗性。由于抗X.指数的葡萄砧木根系甚至连易感品种的根系在寄生后均未显示出可靠的胆汁形成，此外，在未接种的对照植物上经常发现根系增厚，因此使用线虫繁殖率来验证有关对...的敏感性或抵抗力X.索引。对于我们的方法，进行了玻璃小瓶测定，接种后35天，通过对提取后的幼虫进行计数来确定线虫种群。易感砧木品种的繁殖率高，考虑到品种的抗性越强，繁殖率就降低。除了仅着眼于根胆形成之外，还应包括线虫繁殖率，作为抗药性筛选的可靠指标。所描述的测定装置能够通过快速筛选以选择有希望的砧木候选物来评估抗性水平。