Membrane Shape Dynamics-Based Analysis of the Physical Properties of Giant Unilamellar Vesicles Prepared by Inverted Emulsion and Hydration Techniques,Langmuir

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Membrane Shape Dynamics-Based Analysis of the Physical Properties of Giant Unilamellar Vesicles Prepared by Inverted Emulsion and Hydration Techniques
Langmuir ( IF 3.7 ) Pub Date : 2021-02-08 , DOI: 10.1021/acs.langmuir.0c02698
Masamune Morita ₁ , Naohiro Noda ₁

Affiliation

The giant unilamellar vesicle (GUV) is a basic model of the cell membrane that allows for the modulation and control of membrane shape dynamics, which play essential roles in the functions of living cell membranes. However, to properly use these artificial cell-like model systems, we need to understand their physical properties. GUV generation techniques are key technologies in the synthesis of artificial cell-like model systems. However, it is unclear whether GUVs produced by different techniques have the same physical properties. Here, we have investigated the physical properties of GUVs prepared by inverted emulsion and hydration techniques by examining the membrane shape deformation induced by external stimulation with a nonionic surfactant. We reveal differences in the spontaneous curvature of the membrane, the preferred differential area between the inner and outer leaflets of the membrane, and the edge tension of membrane pores between the GUVs prepared using the two distinct techniques.

中文翻译：

基于膜形动力学的反相乳化和水化技术制备的单层巨型囊泡物理性质的分析

巨大的单层囊泡（GUV）是细胞膜的基本模型，它可以调节和控制膜的形状动力学，而这些动力学在活细胞膜的功能中起着至关重要的作用。但是，要正确使用这些类似人工细胞的模型系统，我们需要了解它们的物理特性。GUV生成技术是合成人工细胞样模型系统的关键技术。但是，尚不清楚通过不同技术生产的GUV是否具有相同的物理特性。在这里，我们通过检查由非离子表面活性剂外部刺激引起的膜形状变形，研究了通过反相乳液和水合技术制备的GUV的物理性能。我们揭示了膜的自发曲率的差异，

更新日期：2021-02-23

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