Abstract

In this study, soybean protein nanoparticles (SPN) modified poly(hydroxybutyrate) (PHB) electrospun nanofiber scaffold is fabricated by a facile two-step method combination of PHB electrospinning and SPN evaporation-induced self-assembly. The surface characteristics, structure and properties are investigated by SEM, EDS, XPS, FTIR, XRD, DMA, WCA, tensile test and water uptake capacity measurements. To characterize biodegradability and cytocompatibility, biodegradation tests are performed in simulated body fluid with and without lysozyme and cell cultures are carried out using NIH3T3 mouse fibroblast cells by in vitro studies. The as-obtained SPN-modified PHB nanofiber scaffold presents better cytocompatibility and is more suitable for biomedical applications.

摘要

在这项研究中，大豆蛋白纳米粒子 (SPN) 改性聚羟基丁酸酯 (PHB) 静电纺丝纳米纤维支架是通过 PHB 静电纺丝和 SPN 蒸发诱导自组装的简单两步法组合制造的。通过 SEM、EDS、XPS、FTIR、XRD、DMA、WCA、拉伸试验和吸水能力测量来研究表面特征、结构和性能。为了表征生物降解性和细胞相容性，在有和没有溶菌酶的模拟体液中进行生物降解测试，并通过体外研究使用 NIH3T3 小鼠成纤维细胞进行细胞培养。所获得的 SPN 修饰的 PHB 纳米纤维支架具有更好的细胞相容性，更适合生物医学应用。