Abstract

Tea polysaccharides (TPS) were analyzed and characterized by HPAEC, GPC, FT-IR, AFM, HSQC, and HMBC NMR as well as methylation. The TPS mainly consisted of neutral (F₀, F_0.2, and F_0.3) and acidic polysaccharides (F_0.1) rich in glucuronic acid. Moreover, anti-proliferative activity of the TPS on HeLa cells was investigated. It was found that F₀ exhibited the highest inhibition rate (36.7%) with 200 µg/ml treatment for 48 h. Structural characterization revealed that F₀ had a backbone chain of (1→4)-β-d-Glcp, (1→6)-α-d-Galp, (1→2)-α-l-Rhap and (1→2,4)-α-l-Rhap residues with (1→6)-α-d-Galp and acetyl group side chains.

摘要

通过HPAEC，GPC，FT-IR，AFM，HSQC和HMBC NMR以及甲基化对茶多糖（TPS）进行了分析和表征。TPS主要由富含葡萄糖醛酸的中性（F ₀，F _0.2和F _0.3）和酸性多糖（F _0.1）组成。此外，还研究了TPS对HeLa细胞的抗增殖活性。结果表明，以200 µg / ml处理48 h ，F ₀表现出最高的抑制率（36.7％）。结构表征表明，F ₀的主链为（1→4）-β - d -Glc p，（1→6）-α - d -Gal p，（1→2）-α - l -Rha p和（1→2,4）-α - l -Rha p残基具有（1→6）-α - d -Gal p和乙酰基侧链。