Abstract

This study was aimed at evaluating the influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of Lactococcus lactis Gh1 after drying and during a long-term storage for 120 days. Highest survivability rate after freeze-drying was observed for cells protected in galactose followed by trehalose and peptone. The differences in cell viability among the different types of protective agent and between the concentrations after freeze-drying were not significant. However, significant difference was seen between the protective agents at different concentrations during storage. At high storage temperatures, the number of viable cells decreased for all protective agents and concentrations investigated in this study. Antibacterial activity from L. lactis Gh1 against Listeria monocytogenes ATCC 15313 was in linear correlation with the viable cell counts. Possible formulation of protective agent from this study could be applied in the industrial development of probiotic and bioprotective cultures.

摘要

本研究旨在评估冻干保护剂的类型和浓度、储存温度和储存时间对乳酸乳球菌细胞活力和抗菌活性的影响。Gh1 干燥后和长期储存 120 天。对于在半乳糖中保护的细胞，其次是海藻糖和蛋白胨，观察到冷冻干燥后的最高存活率。不同类型保护剂之间和冻干后浓度之间的细胞活力差异不显着。然而，在储存过程中，不同浓度的保护剂之间存在显着差异。在高储存温度下，对于本研究中研究的所有保护剂和浓度，活细胞的数量都会减少。L. lactis Gh1 对李斯特菌的抗菌活性ATCC 15313 与活细胞计数呈线性相关。本研究中可能的保护剂配方可用于益生菌和生物保护培养物的工业开发。