Abstract

This study aimed to verify the effects of different drying methods and temperatures of fish myofibrillar proteins (MPs), on the technological properties of biodegradable films. The MPs were dried by lyophilization at −60 °C for 48 h and by convective drying of hot air at 40 °C, 60 °C, and 80 °C. In the drying by convection, drying curves were constructed and the adjustments of mathematical models were evaluated. The films were formulated with 1.5% proteins (w/v) and 30% glycerol (w/w), and they were characterized. The drying times found were 540 min (40 °C), 300 min (60 °C), and 120 min (80 °C). The “Logarithmic” models followed by the “Diffusion approach” were the ones that best fit for all temperatures. The results of protein electrophoresis and the characterization of the film showed that the convective drying caused changes in the three-dimensional structure of the proteins, favoring the formation of a highly cross-linked protein network with numerous hydrogen bonds, hydrophobic interactions, and disulfide bonds, and generating films with properties equal to or better than lyophilized proteins.

摘要

本研究旨在验证鱼肌原纤维蛋白 (MPs) 的不同干燥方法和温度对可生物降解薄膜技术性能的影响。通过在 -60 °C 下冻干 48 小时和通过在 40 °C、60 °C 和 80 °C 下对热空气进行对流干燥来干燥 MP。在对流干燥中，建立了干燥曲线并对数学模型的调整进行了评价。薄膜由 1.5% 的蛋白质 (w/v) 和 30% 的甘油 (w/w) 配制而成，并对其进行了表征。发现的干燥时间为 540 分钟 (40 °C)、300 分钟 (60 °C) 和 120 分钟 (80 °C)。“对数”模型和“扩散法”是最适合所有温度的模型。