A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology.

中文翻译：

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用于鼠口腔细菌群落的 16S rRNA 基因和基因组草案数据库

结合细菌培养、16S rRNA 基因扩增子和全基因组测序，构建了一个精心策划的小鼠口腔微生物组数据库，用作基于小鼠的研究的参考。该数据库包含来自培养分离物的近乎全长的 16S rRNA 基因序列的集合，以及来自各种来源的代表性分类群的基因组草图，这些来源包括无特定病原体的实验室小鼠、野生小家鼠 和以前的野生木鼠蚜虫。目前，它包括 103 个小鼠口腔分类群 (MOT)，跨越四个门——厚壁菌门、变形菌门、放线菌门和拟杆菌门——包括 12 个新的未描述的物种级分类群。本研究的主要观察结果是 (i) 实验室小鼠口腔微生物组的低多样性和主要可培养性，以及 (ii) 鉴定出三种主要的鼠特异性口腔细菌谱系，即Streptococcus danielae (MOT10)、Lactobacillus murinus ( MOT93) 和Gemella物种 2 (MOT43)，它是一种新的、仍未命名的分类群。其中，S. danieliae尤其令人感兴趣，因为它是来自所有健康和牙周病实验室小鼠品系的口腔微生物组的主要组成部分，并且存在于野生小鼠中。预计这个特征良好的数据库应该是一个有用的资源口腔微生物学领域的体外实验和小鼠模型研究。