Fast and high-throughput determination of drugs is a key trend in clinical medicine. Single particles have increasingly been adopted in a variety of photoanalytical and electroanalytical applications, and microscopic analysis has been a hot topic in recent years, especially for electrochemiluminescence (ECL). This paper describes a simple ECL method based on single gold microbeads to image lecithin. Lecithin reacts to produce hydrogen peroxide under the successive enzymatic reaction of phospholipase D and choline oxidase. ECL was generated by the electrochemical reaction between a luminol analog and hydrogen peroxide, and ECL signals were imaged by a camera. Despite the heterogeneity of single gold microbeads, their luminescence obeyed statistical regularity. The average luminescence of 30 gold microbeads is correlated with the lecithin concentration, and thus, a visualization method for analyzing lecithin was established. Calibration curves were constructed for ECL intensity and lecithin concentration, achieving detection limits of 0.05 mM lecithin. This ECL imaging platform based on single gold microbeads exhibits outstanding advantages, such as high throughput, versatility and low cost, and holds great potential in disease diagnostics, environmental monitoring and food safety.

药物的快速、高通量测定是临床医学的一个关键趋势。单粒子越来越多地被用于各种光分析和电分析应用中，微观分析一直是近年来的热门话题，尤其是电化学发光 (ECL)。本文介绍了一种基于单个金微珠对卵磷脂进行成像的简单 ECL 方法。卵磷脂在磷脂酶 D 和胆碱氧化酶的连续酶促反应下反应生成过氧化氢。ECL 是由鲁米诺类似物和过氧化氢之间的电化学反应产生的，ECL 信号由相机成像。尽管单个金微珠具有异质性，但它们的发光遵循统计规律。30个金微珠的平均发光度与卵磷脂浓度相关，从而建立了分析卵磷脂的可视化方法。针对 ECL 强度和卵磷脂浓度构建了校准曲线，实现了 0.05 mM 卵磷脂的检测限。这种基于单个金微珠的 ECL 成像平台具有高通量、通用性和低成本等突出优势，在疾病诊断、环境监测和食品安全方面具有巨大潜力。