There are still numerous difficulties in the successful farming of pikeperch in the anthropogenic environment of various aquaculture systems, especially during early developmental steps in the hatchery. To investigate the physiological processes involved on the molecular level, we determined the basal expression patterns of 21 genes involved in stress and immune responses and early ontogenesis of pikeperch between 0 and 175 days post hatch (dph). Their transcription patterns most likely reflect the challenges of growth and feed conversion. The gene coding for apolipoprotein A (APOE) was strongly expressed at 0 dph, indicating its importance for yolk sac utilization. Genes encoding bone morphogenetic proteins 4 and 7 (BMP4, BMP7), creatine kinase M (CKM), and SRY-box transcription factor 9 (SOX9) were highly abundant during the peak phases of morphological changes and acclimatization processes at 4–18 dph. The high expression of genes coding for peroxisome proliferator-activated receptors alpha and delta (PPARA, PPARD) at 121 and 175 dph, respectively, suggests their importance during this strong growth phase of juvenile stages. As an alternative experimental model to replace further in vivo investigations of ontogenetically important processes, we initiated the first approach towards a long-lasting primary cell culture from whole pikeperch embryos. The present study provides a set of possible biomarkers to support the monitoring of pikeperch farming and provides a first basis for the establishment of a suitable cell model of this emerging aquaculture species.

在各种水产养殖系统的人为环境中成功养殖梭鲈仍然存在许多困难，特别是在孵化场的早期发育阶段。为了研究分子水平上涉及的生理过程，我们确定了涉及应激和免疫反应以及梭鲈孵化后 0 至 175 天 (dph) 早期个体发育的 21 个基因的基础表达模式。它们的转录模式很可能反映了生长和饲料转化的挑战。编码载脂蛋白 A ( APOE )的基因在 0 dph 时强烈表达，表明其对于卵黄囊利用的重要性。编码骨形态发生蛋白 4 和 7 ( BMP4、BMP7 )、肌酸激酶 M ( CKM ) 和 SRY-box 转录因子 9 ( SOX9 ) 的基因在 4-18 dph 形态变化和适应过程的高峰期高度丰富。编码过氧化物酶体增殖物激活受体 α 和 δ（ PPARA、PPARD）的基因分别在 121 dph 和 175 dph 时高表达，表明它们在幼鱼阶段的这一强劲生长阶段的重要性。作为替代对个体发生重要过程进行进一步体内研究的替代实验模型，我们提出了第一种从整个梭鲈胚胎中进行持久原代细胞培养的方法。本研究提供了一组可能的生物标志物来支持梭鲈养殖的监测，并为建立这种新兴水产养殖物种的合适细胞模型提供了第一个基础。