Recently, laser radiation and nanomaterials have been utilized to improve biogas yield via anaerobic digestion of herd’s manure through biostimulating methanogenic bacteria. Yet, laser irradiation and nanomaterials as anaerobic bacteria stimulant could have environmental impacts that have not been assessed or known. The aim of the current research was to understand and evaluate variable laser doses in the presence of nickel nanoparticles (Ni NPs) and their environmental impacts during the production of biogas from treated manure. A life cycle assessment scheme was employed to achieve this aim. The used laser doses were 0.5 h, 1 h and 2 h and correlated to 1-h incandescent light exposure, where all treatments received 2 g/m3 Ni NPs. The outcomes were conferred in the pattern of specific influences for the biogas utilization and production as an energy source. The studied impacts were global warming, greenhouse gas emissions mitigation, acidification, eutrophication, ozone layer depletion, freshwater ecotoxicity and prospective human toxicity. Results revealed that laser irradiation with the addition of Ni NPs during the biostimulation of anaerobic digestion has the least environmental adverse effects when compared to the control group.

最近，通过生物刺激产甲烷细菌，通过对牛粪进行厌氧消化，激光辐射和纳米材料已被用于提高沼气产量。然而，激光辐照和作为厌氧细菌刺激物的纳米材料可能会对环境产生影响，而这种影响尚未得到评估或知道。当前研究的目的是了解和评估存在镍纳米颗粒（Ni NPs）时的可变激光剂量及其在处理粪便生产沼气过程中对环境的影响。使用生命周期评估方案来实现此目标。使用的激光剂量分别为0.5 h，1 h和2 h，并与1 h白炽灯照射相关，所有处理均接受2 g / m3 Ni NP。成果以对沼气利用和生产作为能源的具体影响方式产生。研究的影响是全球变暖，减轻温室气体排放，酸化，富营养化，臭氧层消耗，淡水生态毒性和预期的人类毒性。结果显示，与对照组相比，在厌氧消化生物刺激过程中添加Ni NPs进行激光照射对环境的影响最小。