Laryngeal squamous cell carcinoma (LSCC) is one of the most prevalent malignancy of the upper aerodigestive tract. Detection of early lesions in vivo could improve the survival rate significantly. In this study, we demonstrated that femtosecond multiphoton microscopy (MPM) is an effective tool to visualize the microscopic features within fixed laryngeal tissues, without sectioning, staining, or labeling. Accurate detection of lesions and determination of the tumor grading can be achieved, with excellent consistency with conventional histological examination. These results suggest that MPM may represent a powerful tool for in-vivo or fast ex-vivo diagnosis of laryngeal lesions at the point of care.

中文翻译：

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飞秒多光子显微镜对喉部病变进行光学活检

喉鳞状细胞癌（LSCC）是上消化道最普遍的恶性肿瘤之一。体内早期病变的检测可以显着提高生存率。在这项研究中，我们证明了飞秒多光子显微镜（MPM）是一种有效的工具，可以可视化固定喉组织内的微观特征，而无需切片，染色或标记。与常规组织学检查具有极好的一致性，可以实现病变的准确检测和肿瘤分级的确定。这些结果表明，MPM可能是在护理点进行喉部病变的体内或体外快速诊断的有力工具。