Abstract

The chemical characterization study of an endemic plant, Haplanthodes neilgherryensisis (Wight) R.B. Majumdar from Western Ghats of India, resulted in to the isolation of a new flavanone glycoside, 5-hydroxy-7-methoxy-8-O-β-D-glucopyranosyl-2S-flavanone (1), along with 3 known flavonoids, 7-O-methyl dihydrowogonin (2), 7-O-methyl wogonin (3), andrographidine C (4). The structure of 1 was elucidated by using 1 D and 2 D NMR and HRMS experimental data, while for the known compounds, ¹H NMR and mass spectrometry data were compared with the reported literature. Compound 1 was tested in vitro to check the improvement in uptake of glucose by the L6 rat skeletal muscle tissues and the observed EC₅₀ value was 5.8 µM, while rosiglitazone showed EC₅₀ of 2.7 µM.

摘要

对印度西高止山脉 (Western Ghats) 的特有植物Haplanthodes neilgherryensis是 (Wight) RB Majumdar 进行化学表征研究，分离出一种新的黄烷酮糖苷 5-hydroxy-7-methoxy-8-O- β -D-吡喃葡萄糖基-2S-黄烷酮 ( 1 )，以及 3 种已知的黄酮类化合物，7-O-甲基二氢汉黄芩素 ( 2 )、7-O-甲基汉黄芩素 ( 3 )、穿心莲 C ( 4 )。1的结构通过1 D和2 D NMR和HRMS实验数据阐明，而对于已知化合物，¹ H NMR和质谱数据与报道的文献进行了比较。化合物1在体外测试检查L6大鼠骨骼肌组织对葡萄糖摄取的改善，观察到的EC ₅₀值为5.8 µM，而罗格列酮显示EC ₅₀为2.7 µM。