Among bloodsucking arthropods, hard tick is a vector of transmitting the most diverse human and animal pathogens, leading to an increasing number of manifestations worldwide. The development of the anti-tick vaccine has the potential to be an environmentally friendly and cost-effective option for tick management. We have previously demonstrated the induction of both humoral and cellular response against Hyalomma asiaticum (H. asiaticum) following immunization with recombinant cathepsin L-like cysteine protease from H. asiaticum tick (rHasCPL), and could control tick infestations. Interferon-gamma (IFN-γ), is an immunomodulatory factor that plays an important role in the regulation of adaptive immunity against infection. In the present study, recombinant BALB/c mouse IFN-γ (rMus-IFN-γ) was cloned and expressed using a prokaryotic expression system, and verified by Western blotting and IFN-γ-ELISA kit analysis. Female BALB/c mice (n = 12) were used for immunization using rHasCPL (100 μg) plus IFN-γ as adjuvant (10 μg). In immunized female BALB/c mice, the levels of anti-CPL antibodies as well as cytokines were determined using ELISA analysis. Protective efficacy of immunization was evaluated by larvae H. asiaticum challenge of immunized female BALB/c mice. Using rMus-IFN-γ as an adjuvant to rHasCPL vaccine (CPL + IFN-γ) promoted specific antibody IgG (IgG1 > IgG2a) and increased production of IFN-γ and IL-4 compared to immune rHasCPL group (CPL). The protected rate of immunized mice from tick challenge was significantly higher after immunization with CPL + IFN-γ (85.11 %) than with CPL (63.28 %). Immunization using CPL + IFN-γ promoted the activation of anti-HasCPL humoral and cellular immune responses, and could provide better protection against H. asiaticum infestation. This approach may could help develop a candidate vaccine for control tick infestations.

在吸血节肢动物中，硬蜱是传播最多样化的人类和动物病原体的载体，导致全世界越来越多的表现形式。抗蜱疫苗的开发有可能成为一种环境友好且具有成本效益的蜱管理选择。我们之前已经证明了在用来自H. asiaticum 的重组组织蛋白酶 L 样半胱氨酸蛋白酶进行免疫后，可以诱导针对Hyalomma asiaticum ( H. asiaticum ) 的体液和细胞反应。蜱（rHasCPL），并可以控制蜱虫感染。干扰素-γ (IFN-γ) 是一种免疫调节因子，在调节针对感染的适应性免疫中起重要作用。在本研究中，重组 BALB/c 小鼠 IFN-γ（rMus-IFN-γ）使用原核表达系统进行克隆和表达，并通过蛋白质印迹和 IFN-γ-ELISA 试剂盒分析进行验证。使用 rHasCPL (100 μg) 加 IFN-γ 作为佐剂 (10 μg) 使用雌性 BALB/c 小鼠 (n = 12) 进行免疫。在免疫的雌性 BALB/c 小鼠中，使用 ELISA 分析确定了抗 CPL 抗体和细胞因子的水平。用积雪草幼虫评价免疫保护效果免疫的雌性 BALB/c 小鼠的挑战。与免疫 rHasCPL 组 (CPL) 相比，使用 rMus-IFN-γ 作为 rHasCPL 疫苗（CPL + IFN-γ）的佐剂可促进特异性抗体 IgG（IgG1 > IgG2a）并增加 IFN-γ 和 IL-4 的产生。用 CPL + IFN-γ (85.11 %) 免疫接种后，免疫小鼠免受蜱攻击的保护率显着高于 CPL (63.28 %)。使用 CPL + IFN-γ 的免疫促进了抗 HasCPL 体液和细胞免疫反应的激活，并可以提供更好的保护，防止H. asiaticum感染。这种方法可能有助于开发一种用于控制蜱虫感染的候选疫苗。