Ultrasound-assisted extraction (UAE) of antioxidant compounds from Diaporthe schini biomass using ethanol as solvent was performed and the results were compared with heat-assisted extraction (HAE; conventional technique). Ultrasound intensity (17–85 W cm⁻²) and pulse cycle (0.50–1.00) were evaluated according to a Central Composite Rotatable Design (CCRD) to obtain the highest extraction yield. The HAE was studied at 30 and 50 °C. In the validation condition (85 W cm⁻² and 0.93) of the UAE the extraction yield and antioxidant capacity (DPPH) were 22.30 ± 0.47% and 91.35 ± 0.27%, respectively. The best extraction yield (8.34 ± 0.38%) and antioxidant activity (91.32 ± 0.98%) of the HAE were observed at 50 ºC. The GC/MS analysis of the UAE extracts showed the presence of methyl 9,12 octadecadienoate (Z, Z)-, ethyl ester of hexadecanoic acid, ethyl octadec-9-enoate, and 1,4-diaza-2,5-dioxo-3-isobutyl bicyclo[4.3.0]nonane, which could be responsible for the antioxidant activity observed. This study was the first attempt to optimize the UAE parameters for the antioxidant compounds extraction from Diaporthe schini. UAE improved the extraction yield and reduced the extraction time in relation to HAE.

进行了以乙醇为溶剂的白僵菌生物质中抗氧化剂化合物的超声辅助提取（UAE），并将其与热辅助提取（HAE；常规技术）进行了比较。根据中央复合旋转设计（CCRD）对超声强度（17–85 W cm ^-2）和脉冲周期（0.50–1.00）进行了评估，以获得最高的提取率。在30和50°C下研究了HAE。在验证条件下（85 W cm ^-2和0.93）的阿联酋萃取率和抗氧化能力（DPPH）分别为22.30±0.47％和91.35±0.27％。在50ºC下观察到HAE的最佳提取率（8.34±0.38％）和抗氧化活性（91.32±0.98％）。阿联酋提取物的GC / MS分析显示，存在9,12十八碳十二烯酸甲酯（Z，Z）-，十六烷酸乙酯，十八烷基9-烯酸乙酯和1,4-二氮杂2,5-二氧杂环己烷。 -3-异丁基双环[4.3.0]壬烷，可能与观察到的抗氧化活性有关。这项研究是首次尝试优化用于从Diaporthe schini中提取抗氧化剂化合物的阿联酋参数。与HAE相比，阿联酋提高了提取率并减少了提取时间。