Abstract

This work presents a study using coconut cultivation wastes as carbon sources to produce lignocellulosic ethanol, such as green coconut shell (GCS), coconut-tree leaflet (CLL) and coconut-tree leaf stalk (CLS). The biomasses were submitted to hydrothermal (HPT) and acidic (APT) pretreatment. Enzymatic hydrolysis was performed using Cellic® Ctec2 supplemented with 10% Cellic® Htec, mainly based on the identification of glucose and xylose. It was found that enzymatic hydrolysis after HPT promoted better results when compared to APT. The glucose concentrations obtained for the biomasses submitted to the HPT were: 31.85 g/L for the GCS, 21.31 g/L for the CLL and 45.39 g/L for the CLS. For xylose, the higher concentration obtained was 7.93 g/L. During the fermentation step applying the yeast Kluyveromyces marxianus, ethanol concentrations (g/L) from HPT and APT hydrolyzed liquors were: 8.83 and 9.71 g/L for GCS, 10.26 and 7.01 g/L for CLL and 12.99 and 7.44 g/L for CLS, respectively. This study identified the energy potential of coconut residues in the production of biofuels, specifically ethanol, from K. marxianus, species which is little explored commercially and able to ferment glucose and xylose.

Graphic Abstract

中文翻译：

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农业椰子种植废料为马克斯克鲁维酵母生产木质纤维素乙醇的原料

摘要

这项工作提出了一项利用椰子种植废料作为碳源生产木质纤维素乙醇的研究，例如绿色椰子壳（GCS），椰子树小叶（CLL）和椰子树叶茎（CLS）。将生物质进行水热（HPT）和酸性（APT）预处理。酶促水解反应是使用Cellic®Ctec2补充10％Cellic®Htec进行的，主要基于葡萄糖和木糖的鉴定。发现与APT相比，HPT后的酶促水解具有更好的结果。提交给HPT的生物质获得的葡萄糖浓度为：GCS为31.85 g / L，CLL为21.31 g / L，CLS为45.39 g / L。对于木糖，获得的较高浓度为7.93 g / L。在发酵步骤中，使用酵母克鲁维酵母，来自HPT和APT水解液的乙醇浓度（g / L）：GCS为8.83和9.71 g / L，CLL为10.26和7.01 g / L，CLS为12.99和7.44 g / L。这项研究确定了在马克斯克鲁维酵母中生产生物燃料（特别是乙醇）中椰子残渣的潜在能量，该物种在商业上很少开发，并且能够发酵葡萄糖和木糖。

图形摘要