Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a phenolic compound widely used in pharmaceutics and nutraceutics. Although resveratrol is produced by some plant species, including grapes, peanuts, and berries, the content of resveratrol and its derivatives are very low. Therefore, an alternative biosynthetic method using microorganisms, such as Escherichia coli, has been developed over the past two decades. In the present study, a resveratrol-over-producing E. coli strain was developed using three strategies. First, we increased the synthesis of p-coumaric acid, a precursor of resveratrol, by manipulating genes in the shikimate pathway of E. coli. Second, three genes involved in resveratrol biosynthesis, such as tyrosine ammonia lyase (TAL), 4-coumaroyl CoA ligase (4CL), and stilbene synthase (STS), were cloned from diverse sources, such as plants and microorganisms, and the best combination was selected to maximize resveratrol production in E. coli. Finally, culture conditions, such as cell concentration, culture temperature, and carbon sources, were established for optimal resveratrol production. Through these strategies, approximately 80.4 mg/L of resveratrol was biosynthesized after 48 h of culture using glycerol as a carbon source.

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代谢工程化大肠杆菌生物合成白藜芦醇

白藜芦醇（3,5,4'-三羟基-反式-二苯乙烯）是一种广泛用于制药和营养学领域的酚类化合物。尽管白藜芦醇是由一些植物产生的，包括葡萄，花生和浆果，但是白藜芦醇及其衍生物的含量非常低。因此，在过去的二十年中，已经开发出使用微生物例如大肠杆菌的替代生物合成方法。在本研究中，使用三种策略开发了白藜芦醇生产过剩的大肠杆菌菌株。首先，我们通过操纵大肠杆菌sh草酸途径中的基因，增加了白藜芦醇前体对香豆酸的合成。其次，从不同来源克隆了涉及白藜芦醇生物合成的三个基因，例如酪氨酸氨裂合酶（TAL），4-香豆酰CoA连接酶（4CL）和二苯乙烯合酶（STS），例如植物和微生物，并选择最佳组合以使大肠杆菌中白藜芦醇的产量最大化。最后，建立培养条件，例如细胞浓度，培养温度和碳源，以最佳地生产白藜芦醇。通过这些策略，使用甘油作为碳源培养48小时后，生物合成了约80.4 mg / L的白藜芦醇。