Background: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing. Objective: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation. Methods: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level. Results: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds. Conclusion: This system is definitely suitable for women’s point-of-care testing (POCT).

中文翻译：

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基于微流控芯片的手持式变焦显微成像系统用于阴道炎症即时检测 (POCT)

背景：阴道炎是一种常见且非常隐秘的疾病，目前的诊断是经常去医院检查。目的：为了提高检测的便捷性和速度，本文开发了一种基于微流控芯片的手持式变焦显微成像系统，用于阴道炎症即时检测（POCT）。方法：该系统由微流控芯片、光学系统和手持变焦系统与手机组装而成。在硬件方面，我们设计了自吸微流控芯片，可以实现样品的自动取样和充分混合。我们还开发了适用于智能手机的光学系统，其镜头组具有 37 倍放大功能，并配备了对焦范围为 4mm 至 6mm 的变焦系统。在软件方面，我们提出了一个可以准确识别球菌并可以确定炎症水平的APP。结果：与医院观察员的识别率相比，便携式识别系统的检测准确率为95%，经临床样本检测，结果与医院诊断结果完全一致。检出限为500 CFU/ml，相对误差为（0.9±0.3）%，识别时间为7秒。结论：该系统绝对适用于女性即时检测 (POCT)。结果与医院诊断结果完全一致。检出限为500 CFU/ml，相对误差为（0.9±0.3）%，识别时间为7秒。结论：该系统绝对适用于女性即时检测 (POCT)。结果与医院诊断结果完全一致。检出限为500 CFU/ml，相对误差为（0.9±0.3）%，识别时间为7秒。结论：该系统绝对适用于女性即时检测 (POCT)。