Regeneration and reconstruction of segmental bone defects (SBD) is a clinical challenge in maxillofacial surgery and orthopedics. The present study evaluated efficacy of guided bone-regeneration (GBR) of rat femoral SBD using osteoconductive equine-bone (EB) and beta-tricalcium phosphate (beta-TCP) grafts, either with or without platelet-derived growth-factor (PDGF). Following ethical-approval, 50 male Wistar-Albino rats (aged ~12–15 months and weighing ~450–500 g) were included. A 5 mm femoral critical-size SBD was created and animals were divided into five groups depending on the graft material used for GBR (EB, EB + PDGF, Autograft, beta-TCP, beta-TCP + PDGF; n = 10/group). Following 12-weeks of healing, animals were sacrificed and femur specimens were analyzed through qualitative histology and quantitative histomorphometry. There was new bone bridging femoral SBD in all groups and qualitatively, better bone formation was seen in autograft and EB + PDGF groups. Histomorphometric bone-area (BA %) was significantly high in autograft group, followed by EB + PDGF, beta-TCP + PDGF, EB, and beta-TCP groups. Addition of PDGF to EB and beta-TCP during GBR resulted in significantly higher BA%. After 12-weeks of healing, EB + PDGF for GBR of rat femoral segmental defects resulted in new bone formation similar to that of autograft. Based on this study, GBR with EB and adjunct PDGF could be a potential clinical alternative for reconstruction and regeneration of segmental bone defects.

节段性骨缺损（SBD）的再生和重建是颌面外科和骨科的临床挑战。本研究评估了使用骨传导性马骨 (EB) 和 β-磷酸三钙 (β-TCP) 移植物对大鼠股骨 SBD 进行引导骨再生 (GBR) 的功效，无论是否有血小板衍生生长因子 (PDGF) . 在获得伦理批准后，包括 50 只雄性 Wistar-Albino 大鼠（年龄约 12-15 个月，体重约 450-500 克）。创建 5 mm 股骨临界尺寸 SBD，根据用于 GBR 的移植材料将动物分为五组（EB、EB + PDGF、自体移植、β-TCP、β-TCP + PDGF；n = 10/组）。愈合 12 周后，处死动物并通过定性组织学和定量组织形态测定法分析股骨标本。在所有组中都有新的骨桥股骨 SBD，并且定性地，在自体移植和 EB + PDGF 组中观察到更好的骨形成。自体移植组的组织形态测量骨面积 (BA %) 显着高，其次是 EB + PDGF、β-TCP + PDGF、EB 和β-TCP 组。在 GBR 期间将 PDGF 添加到 EB 和 beta-TCP 导致显着更高的 BA%。愈合 12 周后，EB + PDGF 用于大鼠股骨节段性缺损的 GBR 导致新骨形成，类似于自体移植物。基于这项研究，带有 EB 和辅助 PDGF 的 GBR 可能成为重建和再生节段性骨缺损的潜在临床替代方案。