Abstract

This study was aimed at evaluating the influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of Lactococcus lactis Gh1 after drying and during a long-term storage for 120 days. Highest survivability rate after freeze-drying was observed for cells protected in galactose followed by trehalose and peptone. The differences in cell viability among the different types of protective agent and between the concentrations after freeze-drying were not significant. However, significant difference was seen between the protective agents at different concentrations during storage. At high storage temperatures, the number of viable cells decreased for all protective agents and concentrations investigated in this study. Antibacterial activity from L. lactis Gh1 against Listeria monocytogenes ATCC 15313 was in linear correlation with the viable cell counts. Possible formulation of protective agent from this study could be applied in the industrial development of probiotic and bioprotective cultures.

摘要

这项研究旨在评估冻干保护剂的类型和浓度，储存温度和储存时间对乳酸乳球菌细胞活力和抗菌活性的影响。Gh1干燥后可长期保存120天。对于半乳糖，海藻糖和蛋白ept保护的细胞，冻干后观察到最高存活率。不同类型的保护剂之间以及冷冻干燥后的浓度之间的细胞生存力差异不显着。然而，在储存期间，在不同浓度的保护剂之间观察到显着差异。在高存储温度下，本研究中所有保护剂和浓度下活细胞的数量均减少。乳酸乳球菌Gh1对单核细胞增生李斯特菌的抗菌活性ATCC 15313与活细胞计数呈线性相关。这项研究可能的保护剂配方可用于益生菌和生物保护性培养物的工业开发。