We explored the influence of symbiosis with arbuscular mycorrhizal fungi (AMF) on rhizosphere-soil microecology and its relationship with the artemisinin content in Artemisia annua (Artemisia annua L.). Three treatment groups were established: A. annua in symbiosis with Funneliformis mosseae, A. annua in symbiosis with Glomus versiforme, and A. annua without AMF symbiosis as a blank control. The nutrient content, pH, microbial density, activity, diversity, and evenness as well as the urease, acid phosphatase, and sucrase activities of A. annua rhizosphere soil and the relative artemisinin content in A. annua in the absence and presence of AMF symbiosis were measured and analyzed at four stages: T1 (rosette stage, 30 d after transplanting), T2 (growth stage, 70 d after transplanting), T3 (mature stage, 90 d after transplanting), and T4 (harvest stage, 120 d after transplanting). The results showed that AMF symbiosis increased the activities of urease, acid phosphatase, and sucrase in the rhizosphere soil of A. annua; promoted the absorption and utilization of N and P; increased the relative content of artemisinin in the aboveground parts of A. annua; and improved the utilization of various carbon sources by rhizosphere microorganisms to some extent. In addition, AMF also improved the density, quantity. and diversity of microorganisms in the A. annua rhizosphere. Different AMF had different effects on A. annua. The results showed that AMF symbiosis improved the absorption and utilization of soil nutrients by A. annua and increased its relative artemisinin content. These results may provide a theoretical basis for the production of A. annua.

中文翻译：

---

丛枝菌根真菌共生对青蒿根际土壤微生物多样性及酶活性的影响

我们探讨了与丛枝菌根真菌（AMF）共生对根际土壤微生态的影响及其与青蒿（Artemisia annua L.）中青蒿素含量的关系。建立了三个治疗组：青蒿与共生Funneliformis霉，青蒿与共生菌根地表球囊霉和青蒿没有AMF共生作为空白对照。营养成分，pH值，微生物密度，活性，多样性和均匀度以及尿素酶，酸性磷酸酶，和蔗糖酶的活性青蒿根际土壤和在相对青蒿素含量青蒿在不存在和存在 AMF 的情况下，在四个阶段测量和分析共生：T1（莲座期，移植后 30 天），T2（生长阶段，移植后 70 天），T3（成熟期，移植后 90 天）和T4（收获期，移栽后120天）。结果表明，AMF共生提高了青蒿根际土壤中脲酶、酸性磷酸酶和蔗糖酶的活性；促进氮、磷的吸收利用；在增加的地上部分青蒿素的相对含量青蒿; 并在一定程度上提高了根际微生物对各种碳源的利用。此外，AMF还提高了密度、数量。青蒿中微生物的多样性和多样性根际。不同的AMF对青蒿有不同的影响。结果表明，AMF共生提高了青蒿对土壤养分的吸收利用，提高了青蒿素的相对含量。这些结果可为青蒿的生产提供理论依据。