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Upgrade of BL‐5C as a highly automated macromolecular crystallography beamline at Pohang Light Source II
Journal of Synchrotron Radiation ( IF 2.4 ) Pub Date : 2021-02-05 , DOI: 10.1107/s1600577521000588
Jae-Hee Jeong , Cheolsoo Eo , Hyo-Yun Kim , Jin-Hong Kim , Chae-Soon Lee , Hyeong-Joo Choi , Yeon-Gil Kim

BL‐5C is an in‐vacuum undulator beamline dedicated to macromolecular crystallography (MX) at the 3 GeV Pohang Light Source II in Korea. The beamline delivers X‐ray beams with a focal spot size of 200 µm × 40 µm (FWHM, H × V) over the energy range 6.5–16.5 keV. The measured flux is 7 × 1011 photons s−1 at 12.659 keV through an aperture size of 50 µm. The experimental station is newly equipped with the photon‐counting detector EIGER 9M, the multi‐axis micro‐diffractometer MD2, and a robotic sample changer with a high‐capacity dewar. These instruments enable the operation of this beamline as an automated MX beamline specialized in X‐ray fragment screening. This beamline can collect more than 400 data sets a day without human intervention, and a difference map can be automatically calculated by using the data processing pipeline for ligand or fragment identification.

中文翻译:

浦项光源II将BL-5C升级为高度自动化的高分子晶体学光束线

BL-5C是在韩国3 GeV浦项光源II上用于大分子晶体学(MX)的真空起伏射束线。光束线在6.5-16.5 keV的能量范围内发出焦斑尺寸为200 µm×40 µm(FWHM,H×V)的X射线束。测得的通量为7×10 11个 光子s -1在12.659 keV时通过50 µm的孔尺寸。实验站新配备了光子计数检测器EIGER 9M,多轴微衍射仪MD2和具有大容量杜瓦瓶的机器人自动进样器。这些仪器使该光束线能够作为专门用于X射线碎片筛选的自动MX光束线进行操作。该束线每天可以收集400多个数据集,而无需人工干预,并且可以使用数据处理管道来自动识别配体或片段,从而计算出差异图。
更新日期:2021-03-04
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