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Nonlinear reconstruction algorithm for line-scanning microscopy via virtual structured-modulation
Optics Communications ( IF 2.4 ) Pub Date : 2021-02-05 , DOI: 10.1016/j.optcom.2021.126844
Famin Wang , Jiawang Zhao , Yunhai Zhang , Yu Cheng , Wei Huang

Line-scanning microscopy with virtually structured modulation (vLSM) provides an easy, low-cost, axial laminating ability, and phase-artifact-free strategy to achieve super-resolution imaging. In this paper, we apply the nonlinear reconstruction (NLR) algorithm to the vLSM and obtain rapid super-resolution imaging. The reconstruction algorithm can directly process raw data in the spatial domain without complicated processing. NLR algorithm is about 3 times faster than the conventional linear algorithm stemming from the advantage of spatial domain processing, providing 2.02 times the resolution of the wide-field imaging. Both simulated and experimental data were used to verify resolution enhancement.



中文翻译:

虚拟结构调制的线扫描显微镜非线性重建算法

带有虚拟结构调制(vLSM)的线扫描显微镜可提供一种简单,低成本,轴向层压能力以及无相位伪影的策略,以实现超分辨率成像。在本文中,我们将非线性重建(NLR)算法应用于vLSM,并获得了快速的超分辨率成像。该重建算法可以直接在空间域中处理原始数据,而无需进行复杂的处理。NLR算法比传统线性算法快约3倍,这归因于空间域处理的优势,宽视场成像分辨率的2.02倍。模拟和实验数据均用于验证分辨率增强。

更新日期:2021-02-25
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