An efficient microwave-assisted steam distillation followed by a solvent extraction method (MSDE) was established to isolate the essential oil, asarinin and sesamin from the roots and rhizomes of Asarum heterotropoides var. mandshuricum (AHVM). In the MSDE technology applied in this work, moistened roots and rhizomes of AHVM powders are applied to this system. After extracting the essential oil by steam distillation, the powder residue of the roots and rhizomes of AHVM is relatively dry. Hence, this method can save energy consumption, and the loss of asarinin and sesamin can be reduced during the subsequent extraction process and improve essential oil yield. The developed method extracted the essential oil to reach the maximum extraction amount (15.80 ± 0.79 mL/kg) after microwave irradiation for 30 min and maintained the yield balance. Through comparison, MSDE was chosen as the best approach, and under the optimum conditions (90 % aqueous ethanol, liquid–solid ratio of 19.0 mL/g and microwave irradiation power of 332 W) obtained by first order kinetic models and response surface analysis with Box–Behnken design, satisfactory yields of asarinin (1.81 ± 0.72 mg/g) and sesamin (0.74 ± 0.09 mg/g) were achieved. In accordance with the yields of asarinin and sesamin, our research optimized the process parameters. According to the comprehensive comparison of these parameters, the proposed process described in this study has greater advantages than traditional methods. Minimum inhibitory concentrations (MIC_S) of the essential oil, which obtain from the roots and rhizomes of AHVM of five kinds of Fusariums spp. (F. avenaceum, F. trichothecioides, F. sporotrioides, F. sambucinum and F. culmorum) were determination to be 1.25 mg/mL, 1.5 mg/mL, 1.5 mg/mL, > 2 mg/mL and > 2 mg/mL, respectively. The results of the antifungal test showed that the essential oil from the roots and rhizomes of AHVM showed remarkable antifungal activities, in which, F. avenaceum was the most sensitive to the essential oil, with the MIC₅₀ values of 0.625 mg/mL.

建立了一种高效的微波辅助水蒸气蒸馏法，然后采用溶剂萃取法（MSDE）从香辛细辛的根和根茎中分离出香精油，细辛和芝麻素。shu（AHVM）。在这项工作中应用的MSDE技术中，AHVM粉的湿根和根茎被应用于该系统。通过蒸汽蒸馏提取精油后，AHVM的根和根茎的粉末残留物相对干燥。因此，该方法可以节省能量消耗，并且可以减少后续提取过程中天冬酰胺和芝麻素的损失并提高精油产率。所开发的方法在微波照射30分钟后提取精油以达到最大提取量（15.80±0.79 mL / kg），并保持了收率平衡。通过比较，MSDE被选为最佳方法，并且在最佳条件下（乙醇水溶液90％，液固比为19）。通过一级动力学模型和采用Box–Behnken设计的响应面分析获得0 mL / g和332 W的微波辐射功率，令人满意的天冬酰胺（1.81±0.72 mg / g）和芝麻素（0.74±0.09 mg / g）的产量实现了。根据天麻素和芝麻素的收率，我们的研究优化了工艺参数。根据这些参数的综合比较，本研究中描述的拟议过程比传统方法具有更大的优势。最小抑制浓度（MIC 与传统方法相比，本研究中描述的拟议方法具有更大的优势。最小抑制浓度（MIC 与传统方法相比，本研究中描述的拟议方法具有更大的优势。最小抑制浓度（MIC_S）是从五种镰刀菌属AHVM的根和根中获得的精油。（F. avenaceum，F.孢，F. sporotrioides，F.接骨木和F.孢）均确定为1.25毫克/毫升，1.5毫克/毫升，1.5毫克/毫升，> 2毫克/毫升和> 2毫克/ mL，分别。抗真菌试验的结果表明，从根部和AHVM的根茎精油显示出显着的抗真菌活性，其中，F. avenaceum是精油最敏感的，与MIC ₅₀ 0.625 mg / mL的值。