Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high background signals and low selectivity. Bioorthogonal reactions, with the advantages of being both nondestructive and effective, are used to trace and analyze biological interactions in vivo. This review focuses on recent progress in understanding the mechanism of action of fluorescence probes.

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深入了解生物学成像中的荧光成像和生物正交反应

荧光成像是生物医学领域的重要方法。荧光成像是无损的，具有高效率和灵敏度，高分辨率，并允许实时动态监测活细胞。但是，它也有一些缺点，例如背景信号高和选择性低。具有正交性和非破坏性的优点的生物正交反应用于追踪和分析体内的生物相互作用。这篇综述集中在了解荧光探针的作用机理的最新进展。