The ongoing pandemic has demonstrated the utility of widespread surveillance and diagnostic detection of the novel SARS-CoV-2. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) has enabled broader testing, but current LAMP tests only detect single targets and require separate reactions for controls. With flu season in the Northern Hemisphere, the ability to screen for multiple targets will be increasingly important, and the ability to include internal controls in RT-LAMP allows for improved efficiency. Here we describe multiplexed RT-LAMP with four targets (SARS-CoV-2, influenza A, influenza B, human RNA) in a single reaction using real-time and end point fluorescence detection. Such increased functionality of RT-LAMP will enable even broader adoption of this molecular testing approach and aid in the fight against this public health threat.

中文翻译：

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用于检测SARS-CoV-2和流感病毒RNA的多重逆转录环介导的等温扩增的开发。

正在进行的大流行证明了新型SARS-CoV-2的广泛监视和诊断检测的实用性。逆转录环介导的等温扩增（RT-LAMP）可以进行更广泛的测试，但是当前的LAMP测试仅检测单个靶标，并且需要针对对照的单独反应。随着北半球的流感季节，筛选多个目标的能力将变得越来越重要，并且将内部控制纳入RT-LAMP的能力可提高效率。在这里，我们使用实时和终点荧光检测在单个反应中描述了具有四个靶标（SARS-CoV-2，甲型流感，乙型流感，人类RNA）的多重RT-LAMP。