The ongoing pandemic has demonstrated the utility of widespread surveillance and diagnostic detection of the novel SARS-CoV-2. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) has enabled broader testing, but current LAMP tests only detect single targets and require separate reactions for controls. With flu season in the Northern Hemisphere, the ability to screen for multiple targets will be increasingly important, and the ability to include internal controls in RT-LAMP allows for improved efficiency. Here we describe multiplexed RT-LAMP with four targets (SARS-CoV-2, influenza A, influenza B, human RNA) in a single reaction using real-time and end point fluorescence detection. Such increased functionality of RT-LAMP will enable even broader adoption of this molecular testing approach and aid in the fight against this public health threat.

中文翻译：

---

开发多重逆转录环介导的等温扩增用于检测 SARS-CoV-2 和流感病毒 RNA。


持续的大流行证明了对新型 SARS-CoV-2 进行广泛监测和诊断检测的效用。逆转录环介导等温扩增 (RT-LAMP) 实现了更广泛的测试，但目前的 LAMP 测试只能检测单个目标，并且需要单独的反应进行对照。随着北半球流感季节的到来，筛选多个目标的能力将变得越来越重要，并且在 RT-LAMP 中包含内部控制的能力可以提高效率。在这里，我们描述了使用实时和终点荧光检测在单个反应中具有四个靶标（SARS-CoV-2、甲型流感、乙型流感、人类 RNA）的多重 RT-LAMP。 RT-LAMP 功能的增强将使这种分子测试方法得到更广泛的采用，并有助于对抗这一公共卫生威胁。