Purpose

The purpose of this paper is to report on the development of a simple electrochemical nanobiosensor for early detection of pancreatic cancer. The nanobiosensor uses the newly emerged stable micro ribonucleic acid (miR-21) as a cancer-associated biomarker for diagnosis, prognosis or therapy response.

Design/methodology/approach

The biosensing practice consists of two main steps: capturing probe immobilization on a working electrode modified with multi-walled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs) and then sensing the miR-21 interaction electrochemically. Two electrochemical techniques, atomic force microscopy and Fourier-transform infrared spectroscopy, were applied for characterizations.

Findings

The nanobiosensor sensitivity exhibited satisfying results to miR-21 and demonstrated a wide dynamic range with a detection limit of just about 3.68 femtomolar using the source measure unit (SMU).

Originality/value

Researchers commonly use potentiostats to perform the differential pulse voltammetry (DPV) measurements for the electrochemical biosensing applications. In this study, the SMU was used to perform the DPV to detect the biomarker miR-21 using the MWCNTs-AuNPs screen-printed electrode as the electrochemical system.

中文翻译：

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开发用于胰腺癌早期检测的纳米生物传感器

目的

本文的目的是报告用于胰腺癌早期检测的简单电化学纳米生物传感器的开发。纳米生物传感器使用新出现的稳定的微核糖核酸（miR-21）作为癌症相关的生物标志物，用于诊断，预后或治疗反应。

设计/方法/方法

生物传感实践包括两个主要步骤：将探针固定在用多壁碳纳米管和金纳米颗粒（MWCNTs-AuNPs）修饰的工作电极上，然后电化学检测miR-21相互作用。两种电化学技术，原子力显微镜和傅里叶变换红外光谱法，用于表征。

发现

纳米生物传感器的灵敏度对miR-21表现出令人满意的结果，并显示了宽动态范围，使用源测量单元（SMU）的检出限仅为3.68飞摩尔。

创意/价值

研究人员通常使用恒电位仪来进行电化学生物传感应用的差分脉冲伏安法（DPV）测量。在这项研究中，使用MWCNTs-AuNPs丝网印刷电极作为电化学系统，使用SMU进行DPV检测生物标志物miR-21。