The Cas9 nuclease from Streptococcus pyogenes (SpCas9) is the most popular enzyme for CRISPR technologies. However, considering the wide diversity of microorganisms (discovered and still unknown), a massive number of CRISPR effectors are being and will be identified and characterized in the search of optimal Cas variants for each of the many applications of CRISPR. In this context, a versatile and efficient multicellular system for CRISPR editing such as Caenorhabditis elegans would be of great help in the development of these effectors. Here, we highlight the benefits of using C. elegans for the rapid evaluation of new CRISPR effectors, and for optimizing CRISPR efficiency in animals in several ways such as by modulating the balance between repair pathways, modifying chromatin accessibility, or controlling the expression and activity of nucleases and guide RNAs.

中文翻译：

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CRISPR 工具箱中的活生物体：秀丽隐杆线虫是开发 CRISPR-Cas 技术的快速有效模型


来自化脓性链球菌的 Cas9 核酸酶 (SpCas9) 是 CRISPR 技术中最常用的酶。然而，考虑到微生物的广泛多样性（已发现的和仍然未知的），大量的 CRISPR 效应子正在并将在为 CRISPR 的众多应用寻找最佳 Cas 变体的过程中被识别和表征。在这种情况下，用于 CRISPR 编辑的多功能且高效的多细胞系统（例如秀丽隐杆线虫）将对这些效应器的开发有很大帮助。在这里，我们重点介绍使用线虫快速评估新 CRISPR 效应子以及通过多种方式优化动物 CRISPR 效率的好处，例如调节修复途径之间的平衡、改变染色质可及性或控制表达和活性核酸酶和向导RNA。