Ovarian cancer (OC) is the one of the most common cancer in women globally. However, it still represents the most dangerous gynecologic malignancy even with the advances in detection and therapeutics. Thus, there is an urgent need in finding more effective therapeutic options for OC patients including cancer stem cells (CSC). MicroRNAs (miRNAs) are small, endogenous, and non-coding RNAs that play critical roles in the progression of various types of tumor. Our aim of this study was to find the regulatory function of microRNA-26 (miRNA-26b) on the cell proliferation and apoptosis of ovarian CSCs. Our studies show that miR-26b is under-regulated in human CD117+CD44+ ovarian CSCs. The miR-26b overexpression inhibits the cell proliferation and promotes cell apoptosis. Moreover, phosphatase and tensin homolog (PTEN) is found to be a functional target of miR-26b. Moreover, PTEN overexpression reversed the effects of miR-26b on the cell proliferation and apoptosis. PTEN overexpression remarkably accelerated the cell proliferation, and inhibited cell apoptosis. These results indicate that miR-26b regulates cell proliferation and apoptosis of CD117+CD44+ ovarian CSCs by targeting PTEN.>

卵巢癌（OC）是全球女性最常见的癌症之一。然而，即使检测和治疗方法取得进步，它仍然是最危险的妇科恶性肿瘤。因此，迫切需要为 OC 患者寻找更有效的治疗选择，包括癌症干细胞 (CSC)。MicroRNA (miRNA) 是小型、内源性非编码 RNA，在各种类型肿瘤的进展中发挥着关键作用。本研究的目的是寻找 microRNA-26 (miRNA-26b) 对卵巢 CSC 细胞增殖和凋亡的调节功能。我们的研究表明，miR-26b 在人 CD117+CD44+ 卵巢 CSC 中表达不足。miR-26b过表达抑制细胞增殖并促进细胞凋亡。此外，磷酸酶和张力蛋白同源物 (PTEN) 被发现是 miR-26b 的功能靶标。此外，PTEN过表达逆转了miR-26b对细胞增殖和凋亡的影响。PTEN过表达显着加速细胞增殖，并抑制细胞凋亡。这些结果表明，miR-26b 通过靶向 PTEN 来调节 CD117+CD44+ 卵巢 CSC 的细胞增殖和凋亡。>