Esteya vermicola is a potential biological agent of the pinewood nematode (PWN), Bursaphelenchus xylophilus, due to its high infectivity. However, knowledge of E. vermicola colonization in the host pine tree is less known. To reveal the distribution pattern of E. vermicola inside the pine tree and the interactions between fungus and PWN, biocontrol tests were conducted on 10 years old Pinus densiflora. A green fluorescence protein (GFP)‐tagged E. vermicola was used to observe the fungal hyphae. Real‐time TaqMan PCR quantification was applied to quantify the fungal hyphae in host tree. The results suggest that inoculation with the fungus significantly improved the survival rate of tested trees. Besides, the number of PWN extracted from fungal inoculated tested trees significantly decreased. Moreover, the fungal hyphae and lunate conidia grown in tested trees were observed with the GFP‐tagged E. vermicola. The real‐time quantification of E. vermicola illustrated the differential distribution of E. vermicola in wilted, wilting and healthy pine trees. Additionally, the promotion of fungal hyphal dispersion by B. xylophilus was found in the pine trees artificially infected with PWN. This study reveals the distribution of E. vermicola and PWN during biocontrol of pine wilt disease and provides direct evidence of the effectiveness of this fungus to control PWN. The migration of PWN infected by E. vermicola was supposed to improve the fungal extension in host pine tree.

中文翻译：

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松材线虫的迁移促进了松树中Esteya vermicola的定殖

Esteya vermicola由于其高感染性，是松木线虫（PWN）Bursaphelenchus xylophilus的潜在生物因子。但是，关于寄主松树中ver曲菌定植的知识鲜为人知。为了揭示松树内ver线虫的分布模式以及真菌和PWN之间的相互作用，对10岁的松果（Pinus densiflora）进行了生物防治测试。带有绿色荧光蛋白（GFP）标签的E. vermicola用来观察真菌的菌丝。实时TaqMan PCR定量用于定量宿主树中的真菌菌丝。结果表明，用真菌接种显着提高了被测树木的存活率。此外，从真菌接种的试验树中提取的PWN的数量显着减少。此外，用GFP标记的E. vermicola观察到了在测试树木中生长的真菌菌丝和月子分生孢子。ver木的实时定量显示了ver木在枯萎，枯萎和健康的松树中的差异分布。此外，木糖双歧杆菌促进真菌菌丝的分散是在人工感染PWN的松树中发现的。这项研究揭示了松树枯萎病生物防治过程中ver粉菌和PWN的分布，并提供了这种真菌控制PWN有效性的直接证据。被ver虫感染的PWN的迁移被认为可以改善寄主松树中的真菌扩展。