Existing molecular methods of sex identification have mixed success in different classes of birds. We report a new multiplex PCR method for sex identification based on CHD1gene using the P8/P2 and the 2550F/2718R primers. The new multiplex PCR approach successfully identified sex (two bands in female, single band in male) in edible-nest swiftlet (ENS) (Aerodramus fuciphagus inexpectatus) and plume-toed swiftlet (PTS) (Collocalia affinis). The new approach required agarose gel electrophoresis to resolve multiplex PCR products. The simplicity, speed and low cost make this method an adaptable molecular tool for sex identification in ENS and PTS with a possibility in other bird species.

现有的性别鉴定分子方法在不同类别的鸟类中取得了不同的成功。我们报告了一种新的用于基于CHD1基因的性别鉴定的多重PCR方法，使用P8 / P2和2550F / 2718R引物。新的多重PCR方法成功地确定了可食用巢金丝燕（ENS）（Aerdramus fuciphagus inexpectatus）和羽趾金丝雀（PTS）（Colllocalia affinis）的性别（雌性有两个条带，雄性有单个条带）。新方法需要琼脂糖凝胶电泳来解析多重PCR产物。简单，快速和低成本使该方法成为适用于ENS和PTS性别识别的分子工具，在其他鸟类中也可能使用。