This study aims to estimate the material properties of the porcine vitreous while testing it in close to its natural physiological conditions. Eighteen porcine eyes were tested within 48 h post-mortem. A custom-built computer-controlled test rig was designed to support, load and monitor the behaviour of eye globes while being subjected to dynamic rotation cycles mimicking saccade eye movement. Specimens were glued to the base of a container, surrounded by gelatin, frozen and cut in half to expose the vitreous. After thawing, the container was subjected to concentric dynamic rotations of up to 5°, 10° or 15°, while taking 50 MP photos of the specimen every 2 ms. The images were analysed by a digital image correlation algorithm to trace the movement of marked points on the vitreous surface with different radii from the centre of the posterior chamber. The initial camera image was used in building a finite-element model of the test set-up, which was used in an inverse analysis exercise to estimate the material properties of the vitreous. Angular displacements of the monitored points were up to 3.3°, 4.1° and 3.9° in response to eye rotations of 5°, 10° and 15°, respectively. With the experimental relationships between eye rotation and angular displacements used as target behaviour, the inverse analysis exercise estimated the initial shear modulus, the long-term shear modulus and the viscoelastic decay constant of the porcine vitreous as 2.10 ± 0.15 Pa, 0.50 ± 0.04 Pa and 1.20 ± 0.09 s⁻¹, respectively. Consideration of the viscoelasticity of the vitreous was essential to represent its experimental behaviour. Testing the vitreous in close to its normal physiological conditions produced estimations of the initial shear modulus and long-term shear modulus that were, respectively, smaller and larger than reported values (Zimberlin et al. 2010 Soft Matter 6, 3632–3635. (doi:10.1039/b925407b), Liu et al. 2013 J. Biomech. 46, 1321–7. (doi:10.1016/j.jbiomech.2013.02.006), Rossi et al. 2011 Invest. Ophthalmol. Vis. Sci. 52, 3994–4002. (doi:10.1167/iovs.10-6477)).

本研究旨在估计猪玻璃体的材料特性，同时在接近其自然生理条件下对其进行测试。在死后 48 小时内对 18 只猪眼进行了测试。一个定制的计算机控制的测试台被设计用来支持、​​加载和监测眼球的行为，同时进行模拟眼跳的动态旋转循环。将标本粘在容器底部，周围有明胶，冷冻并切成两半，露出玻璃体。解冻后，容器经受高达 5°、10° 或 15° 的同心动态旋转，同时每 2 ms 拍摄 50 MP 的样本照片。通过数字图像相关算法分析图像，以追踪玻璃体表面上不同半径的标记点从后房中心的移动。初始相机图像用于构建测试装置的有限元模型，该模型用于逆分析练习以估计玻璃体的材料特性。监测点的角位移分别为 3.3°、4.1° 和 3.9°，以响应 5°、10° 和 15° 的眼睛旋转。以眼球旋转和角位移之间的实验关系作为目标行为，逆分析估计猪玻璃体的初始剪切模量、长期剪切模量和粘弹性衰减常数分别为 2.10 ± 0.15 Pa、0.50 ± 0.04 Pa和 1.20 ± 0.09 秒 初始相机图像用于构建测试装置的有限元模型，该模型用于逆分析练习以估计玻璃体的材料特性。监测点的角位移分别为 3.3°、4.1° 和 3.9°，以响应 5°、10° 和 15° 的眼睛旋转。以眼球旋转和角位移之间的实验关系作为目标行为，逆分析估计猪玻璃体的初始剪切模量、长期剪切模量和粘弹性衰减常数分别为 2.10 ± 0.15 Pa、0.50 ± 0.04 Pa和 1.20 ± 0.09 秒 初始相机图像用于构建测试装置的有限元模型，该模型用于逆分析练习以估计玻璃体的材料特性。监测点的角位移分别为 3.3°、4.1° 和 3.9°，以响应 5°、10° 和 15° 的眼睛旋转。以眼球旋转和角位移之间的实验关系作为目标行为，逆分析估计猪玻璃体的初始剪切模量、长期剪切模量和粘弹性衰减常数分别为 2.10 ± 0.15 Pa、0.50 ± 0.04 Pa和 1.20 ± 0.09 秒 监测点的角位移分别为 3.3°、4.1° 和 3.9°，以响应 5°、10° 和 15° 的眼睛旋转。以眼球旋转和角位移之间的实验关系作为目标行为，逆分析估计猪玻璃体的初始剪切模量、长期剪切模量和粘弹性衰减常数分别为 2.10 ± 0.15 Pa、0.50 ± 0.04 Pa和 1.20 ± 0.09 秒 监测点的角位移分别为 3.3°、4.1° 和 3.9°，以响应 5°、10° 和 15° 的眼睛旋转。以眼球旋转和角位移之间的实验关系作为目标行为，逆分析估计猪玻璃体的初始剪切模量、长期剪切模量和粘弹性衰减常数分别为 2.10 ± 0.15 Pa、0.50 ± 0.04 Pa和 1.20 ± 0.09 秒^-1，分别。考虑玻璃体的粘弹性对于代表其实验行为至关重要。在接近其正常生理条件下测试玻璃体产生的初始剪切模量和长期剪切模量的估计值分别小于和大于报告值（Zimberlin et al . 2010 Soft Matter 6, 3632–3635. (doi :10.1039/b925407b), Liu et al . 2013 J. Biomech. 46, 1321–7. (doi:10.1016/j.jbiomech.2013.02.006), Rossi et al . 2011 Invest. Ophthalmol. Vis. Sci. 52, 3994–4002。（doi：10.1167/iovs.10-6477））。