The accuracy of an electron microscope depends on the magnification and resolution of the microscope. Hence in order to achieve high accuracy measurement results from the electron microscopic examination, a standard specimen is required to calibrate the transmission electron microscope (TEM). In the present work, gold nanoparticles of uniform size with narrow particle size distribution were synthesized with an aim to use them as reference material (RM) for TEM/high resolution TEM calibration and then release them in market as Bhartiya Nideshak Dravya (BND). As synthesized gold nanoparticles at 20 ± 2 °C temperature and 45± 5% relative humidityand the standard sample were characterized by using HRTEM, model: FEI-Tecnai F30 G2 STWIN at suitable magnification and areas. Both images exhibit spherical shaped nanoparticles with size varying in the range from (15.35-20.15 nm) and (5.10 -7.70 nm) respectively. Stability in particles size of the chemically synthesized gold nanoparticles was studied for a period of one year time span using UV-Visible spectrometer. TEM/HRTEM measurements were carried out in automated mode under similar experimental and environmental conditions at different magnifications to get accurate particle size. Overall uncertainty is estimated in small and big size particles as per standard GUM document guidelines.

中文翻译：

---

用于TEM / HRTEM校准的化学合成的稳定均匀大小的金纳米粒子的不确定度测量

电子显微镜的精度取决于显微镜的放大率和分辨率。因此，为了从电子显微镜检查中获得高精度的测量结果，需要标准样品来校准透射电子显微镜（TEM）。在目前的工作中，合成了具有窄粒度分布的均一尺寸的金纳米粒子，目的是将它们用作TEM /高分辨率TEM校准的参考材料（RM），然后以Bhartiya Nideshak Dravya（BND）的形式投放市场。作为在20±2°C温度和45±5％相对湿度下合成的金纳米粒子，并使用HRTEM对标准样品进行表征，型号：FEI-Tecnai F30 G2 STWIN在适当的放大倍数和面积下。两个图像均显示球形纳米颗粒，其尺寸分别在（15.35-20.15 nm）和（5.10 -7.70 nm）范围内变化。使用紫外可见分光光度计研究了化学合成金纳米粒子在一年时间范围内的粒度稳定性。TEM / HRTEM测量是在相似的实验和环境条件下以不同的放大倍数以自动化模式进行的，以获取准确的粒径。根据标准GUM文档指南，估计大小颗粒中的总体不确定性。TEM / HRTEM测量是在相似的实验和环境条件下以不同的放大倍数以自动化模式进行的，以获取准确的粒径。根据标准GUM文档指南，估计大小颗粒中的总体不确定性。TEM / HRTEM测量是在相似的实验和环境条件下以不同的放大倍数以自动化模式进行的，以获取准确的粒径。根据标准GUM文档指南，估计大小颗粒中的总体不确定性。