With the increased metabolic diversity and adaptability, microorganisms are capable of living in diverse environmental conditions. Various microorganisms present in natural environment are resistant to some of the current antibiotics that has lead to discovery of new antibiotic compounds. Soil organisms have been paid a great attention and are recognized as a good source of antibiotic compounds. Actinomycetes are well known for their importance as bioactive metabolites in different sectors. Present study was carried out via isolation of actinomycetes from rhizospheric soil from four different locations of Rajasthan and the isolation was performed on Actinomycetes Isolation Agar (AIA) medium. In this study AIA10 isolate was screened for their antimicrobial potential against five selected microbial pathogens viz. Staphyloccocus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus vulgaris and Bacillus subtilis. After Solvent extraction, crude extracts of benzene and ethyl acetate were tested for antibacterial activity. GC-MS technique identified the presence of major components like Heptadecane at retention time (RT) - 18.131 (5.07 %), Heneicosane at RT - 20.935 (14.63 %), Hexadecanoic acid, Butyl ester at RT - 25.611 (4.92 %), Eicosanoic acid, 2,3-Bis(Acetyloxy)Propyl ester at RT - 32.509 (8.15 %), 9-Octadecenoic acid (Z)-, 2,3-Bis(Acetyloxy)Propyl ester at RT - 34.978 (34.32 %) in benzene crude extract. In ethyl acetate extract major compounds identified were, Pentadecanoic acid at RT - 6.25 (21.251 %), N-Hexadecanoic acid at RT - 22.527 (12.79 %), Eicosanoic acid at RT - 23.788 (14.18 %), Hexadecanoic acid, 2-Hydroxy-1-(Hydroxymethyl) Ethyl ester at RT - 29.593 (5.59 %), Octadecanoic acid, 2,3-Dihydroxypropyl ester at RT - 33.182 (3.50 %) and 5,11,17, 23-Tetratert Butyl pentacyclo [19.3.1.1~3,7~.1~9,13~.1~15,19~]Octacosa1(25),3(28),4,6,9(27),10,12,15(26),16,18,21, 23-Dodecaene-25,26,27,28 Tetrol at RT - 54.152 (9.92 %). Primary screening was tested and the activity was observed against S. aureus and P. vulgaris. In secondary screening, benzene and ethyl acetate extract was found active against S. aureus, at inhibition zone IZ = 10 and 20 mm, respectively. The study conducted proposes these actinomycetes as a potent source for antimicrobial metabolites.

中文翻译：

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放线菌分离的抗菌药物作为多药耐药生物有效抑制剂的评价

随着新陈代谢多样性和适应性的增加，微生物能够生活在各种环境条件下。自然环境中存在的各种微生物对某些当前的抗生素具有抗性，这导致发现了新的抗生素化合物。土壤生物已受到高度重视，并被认为是抗生素化合物的良好来源。放线菌作为其在不同领域的生物活性代谢物的重要性而闻名。目前的研究是通过从拉贾斯坦邦四个不同地点的根际土壤中分离放线菌进行的，并在放线菌隔离琼脂（AIA）培养基上进行了分离。在这项研究中，对AIA10分离株针对五种选定的微生物病原体的抗微生物潜力进行了筛选，即。金黄色葡萄球菌，铜绿假单胞菌，肺炎克雷伯菌，寻常变形杆菌 和枯草芽孢杆菌。溶剂萃取后，测试苯和乙酸乙酯的粗萃取物的抗菌活性。GC-MS技术鉴定了主要成分的存在，例如保留时间（RT）的庚烷-18.131（5.07％），RT的二十碳烷-20.935（14.63％），十六烷酸，RT的丁酯-25.611（4.92％），二十碳烷酸酸，室温下的2,3-双（乙酰氧基）丙酸酯-32.509（8.15％），室温下的9-十八碳烯酸（Z）-，室温下的2,3-双（乙酰氧基）丙酸酯-苯中的34.978（34.32％）粗提物。在乙酸乙酯提取物中鉴定出的主要化合物为RT时的十五烷酸-6.25（21.251％），RT时的正十六烷酸-RT-22.527（12.79％），RT时的二十烷酸-23.788（14.18％），十六烷酸，2-羟基室温下-1-（羟甲基）乙酯-29.593（5.59％），十八酸，室温下2,3-二羟丙酯-33.182（3.50％）和5,11,17，23-四叔丁基五环[19.3.1.1〜3,7〜.1〜9,13〜.1〜15,19〜] Octacosa1（25），3（28），4,6,9（27），10， 12,15（26），16,18,21，23-十二碳烯25,26,27,28室温下的四醇-54.152（9.92％）。测试了初步筛选，并观察了针对金黄色葡萄球菌和寻常葡萄球菌。在二次筛选中，发现苯和乙酸乙酯提取物分别对抑制区IZ = 10和20 mm具有抗金黄色葡萄球菌的活性。进行的研究提出，这些放线菌是抗菌代谢产物的有效来源。