Impacts of bioturbation by worms on remediation of diesel contaminated sediments were studied in a laboratory experiment. The total petroleum hydrocarbons (TPH) were represented by the oil or diesel particles which were detected in seawater as well as in the upper, middle and lower layer of sediments at every three days of experiment. The whole experiment was lasted for 21 days and the TPH in the worms was measured by fluorescence analysis and the components of residual oil in the sediments were detected by gas chromatography-mass spectrometry (GC-MS) at the end of the experiment. The results showed that the TPH were declined to 18.37±0.62 and 20.85±1.74 mg/kg in the surface and sub-surface sediments, respectively, which were the worms’ frequently active areas. TPH was found to increase slightly in deeper sediments as well as in the water column because of bioturbation by worms. The feeding behavior of worms led to the accumulation of TPH in the worm’s body and was reached from 0.998±0.171 to 23.764±3.878 mg/kg. Many components of the diesel such as alkanes, Polycyclic Aromatic Hydrocarbons (PAHs) etc. were removed through bioturbation by worms. In conclusion, the petroleum hydrocarbons in the area where the worms are located will decrease over time.

中文翻译：

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蠕虫（Perinereis sp。）生物扰动修复柴油污染的沉积物

在实验室实验中研究了蠕虫对生物扰动对柴油污染沉积物的修复作用。每三天在海水中以及沉积物的上层，中层和下层中检测到的油或柴油颗粒​​代表总石油烃（TPH）。整个实验持续了21天，在实验结束时，通过荧光分析法测定了蠕虫中的TPH，并通过气相色谱-质谱法（GC-MS）检测了沉积物中的残留油成分。结果表明，蠕虫最活跃的表层和亚表层沉积物中的TPH分别降至18.37±0.62和20.85±1.74 mg / kg。由于蠕虫的生物扰动，TPH在较深的沉积物以及水柱中略有增加。蠕虫的摄食行为导致TPH在蠕虫体内积聚，达到0.998±0.171至23.764±3.878 mg / kg。柴油机的许多成分，例如烷烃，多环芳烃（PAH）等，都是通过蠕虫通过生物扰动去除的。总之，蠕虫所在区域的石油碳氢化合物将随着时间的流逝而减少。