We investigate skin optical clearing in laboratory animals ex vivo and in vivo by means of low-molecular-weight paramagnetic contrast agents used in magnetic resonance imaging (MRI) and a radiopaque agent used in computed tomography (CT) to increase the sounding depth and image contrast in the methods of fluorescence laser imaging and optical coherence tomography (OCT). The diffusion coefficients of the MRI agents Gadovist, Magnevist, and Dotarem, which are widely used in medicine, and the Visipaque CT agent in ex vivo mouse skin, are determined from the collimated transmission spectra. MRI agents Gadovist and Magnevist provide the greatest optical clearing (optical transmission) of the skin, which allowed: 1) an almost 19-fold increase in transmission at 540 nm and a 7 – 8-fold increase in transmission in the NIR region from 750 to 900 nm; 2) a noticeable improvement in OCT images of skin architecture; and 3) a 5-fold increase in the ratio of fluorescence intensity to background using TagRFP-red fluorescent marker protein expressed in a tumour, after application to the skin of animals in vivo for 15 min. The obtained results are important for multimodal imaging of tumours, namely, when combining laser fluorescence and OCT methods with MRI and CT, since the contrast agents under study can simultaneously enhance the contrast of several imaging methods.

我们通过磁共振成像 (MRI) 中使用的低分子量顺磁造影剂和计算机断层扫描 (CT) 中使用的不透射线剂来研究体外和体内实验动物的皮肤光学清除，以增加探测深度和图像对比荧光激光成像和光学相干断层扫描 (OCT) 的方法。医学上广泛使用的 MRI 试剂 Gadovist、Magnevist 和 Dotarem 以及离体Visipaque CT 试剂的扩散系数小鼠皮肤，由准直透射光谱确定。MRI 试剂 Gadovist 和 Magnevist 可提供最大的皮肤光学清除（光学透射），这使得：1) 在 540 nm 处的透射率增加了近 19 倍，在 NIR 区域的透射率从 750 增加了 7-8 倍至 900 纳米；2）皮肤结构的OCT图像显着改善；和 3)在体内应用于动物皮肤后，使用在肿瘤中表达的 TagRFP-红色荧光标记蛋白，荧光强度与背景的比率增加了 5 倍15分钟。获得的结果对于肿瘤的多模态成像很重要，即当将激光荧光和 OCT 方法与 MRI 和 CT 相结合时，因为所研究的造影剂可以同时增强几种成像方法的对比度。