Based on the combined use of polymerisable polyacrylamide, collagen and an aqueous solution of Flavin adenine dinucleotide (FAD), we have developed a new technique for fabrication of composite phantoms mimicking fluorescence properties of human skin. A comparative analysis of the absorption and scattering coefficients, refractive indices, as well as fluorescence spectra of phantoms with different concentrations of FAD measured with the combined use of a CCD spectrometer and a hyperspectral camera is presented. To obtain values of scattering coefficients close to those for human skin, zinc oxide (ZnO) nanoparticles are added to the polyacrylamide polymer structure. Variations both in the shape of the spectrum and in the intensity of the fluorescence signal in the phantoms are provided by the changes in the volume fraction of FAD and collagen. It is shown that the model fluorescence spectra are in good agreement with the results of direct human skin measurements in vivo.

基于可聚合聚丙烯酰胺、胶原蛋白和黄素腺嘌呤二核苷酸 (FAD) 水溶液的组合使用，我们开发了一种制造模拟人体皮肤荧光特性的复合体模的新技术。对结合使用CCD光谱仪和高光谱相机测量的不同FAD浓度的体模的吸收和散射系数、折射率以及荧光光谱进行了比较分析。为了获得接近人体皮肤的散射系数值，将氧化锌 (ZnO) 纳米粒子添加到聚丙烯酰胺聚合物结构中。FAD 和胶原蛋白的体积分数的变化提供了光谱形状和模型中荧光信号强度的变化。在体内。