Analytical expression for the fluorescence response of a photosensitiser uniformly distributed in the superficial layer of biotissue is obtained in the diffusion approximation of radiative transfer theory, and the approach for estimating the fluorescent layer thickness based on dual-wavelength excitation of fluorescence is proposed. It is shown that the error in estimation of the fluorescent layer thickness employing the ratio of the fluorescence signals obtained at different excitation wavelengths does not exceed 30 % for the thickness range of 0.1 – 2 mm in the case of 30 %-variation of biotissue optical properties.

中文翻译：

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用双波长荧光成像估计生物组织中荧光团定位的荧光强度分析模型

在辐射转移理论的扩散近似下，得到了均匀分布在生物组织表层的光敏剂的荧光响应的解析表达式，提出了基于荧光双波长激发的荧光层厚度估计方法。结果表明，在 30% 的生物组织光学变化的情况下，在 0.1 – 2 mm 的厚度范围内，使用在不同激发波长下获得的荧光信号的比率来估计荧光层厚度的误差不超过 30%特性。