To develop an ex- vivo perfusion flow model using a bovine kidney for future testing of embolic agents in an inexpensive and easy way. Six bovine adult kidneys were used for this study. Kidneys were cannulated and perfused via a roller pump. Three embolic agents, coils, Gelfoam, and a glue mixture of Histoacryl + Lipiodol, were deployed by targeting three secondary segmental arteries per kidney via a 5Fr catheter under fluoroscopic guidance. Cannulation time, success rate of segmental artery selection and embolic agent deployment, total operational time, and fluoroscopy dose were recorded. Average kidney weight was 0.752 +/− 0.094 kg. All six bovine kidneys were successfully cannulated in 21.6 min +/− 3.0 min. Deployment of coils and glue was achieved in every case (12/12); however, Gelfoam injection was not successful in one instance (5/6, 83%). Coil deployment demonstrated no embolic effect while Gelfoam and glue injections demonstrated decreased distal contrast filling post-embolization. Mean dose area product was 12.9 ± 1.8 Gy·cm2, fluoroscopy time was 10 ± 4 min and operational time was 27 ± 8 min. We describe the creation of an ex vivo bovine kidney flow model for the preclinical evaluation of different embolic materials. The flow model can be modified to provide extensive bench testing and it is a promising tool for hands -on training in basic and advanced embolization techniques .

中文翻译：

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建立用于测试栓塞剂的离体牛肾血流模型：正在进行中

要开发一种使用牛肾的体外灌注流模型，以便将来以便宜且简便的方式测试栓塞剂。六个牛成年肾脏用于这项研究。肾插管并通过滚子泵灌注。通过在荧光镜引导下通过5Fr导管将每个肾脏的三个次级节段动脉作为目标，部署了三种栓塞剂，线圈，明胶泡沫和Histoacryl + Lipiodol的胶混合物。记录插管时间，节段动脉选择和栓塞剂部署成功率，总手术时间和透视检查剂量。平均肾脏重量为0.752 +/- 0.094kg。在21.6分钟+/- 3.0分钟内成功插入了所有六个牛肾。在每种情况下都可以展开线圈和胶水（12/12）；但是，一次注射Gelfoam失败（5/6，83％）。线圈展开未显示栓塞作用，而Gelfoam和胶水注射显示栓塞后远端造影剂填充减少。平均剂量面积乘积为12.9±1.8 Gy·cm2，透视时间为10±4分钟，操作时间为27±8分钟。我们描述了用于不同栓塞材料的临床前评估的离体牛肾脏血流模型的创建。可以修改流模型以提供广泛的基准测试，它是在基础和高级栓塞技术上进行动手训练的有前途的工具。我们描述了用于不同栓塞材料的临床前评估的离体牛肾脏血流模型的创建。可以修改流模型以提供广泛的基准测试，它是在基础和高级栓塞技术上进行动手训练的有前途的工具。我们描述了用于不同栓塞材料的临床前评估的离体牛肾脏血流模型的创建。可以修改流模型以提供广泛的台架测试，并且它是用于基础和高级栓塞技术的动手培训的有前途的工具。