Background:Open heart surgery is performed with the aid of cardiopulmonary bypass (CPB) techniques that may cause neuronal injuries. Objective:This study investigated the potential protective effect of oleocanthal pre-treatment against CPB-induced cerebral injury. Methods:Oleocanthal 30 mg/kg i.p.was administered 3 h before CPB induction in the treated group. Behavioral neurological scores and cerebral injury were assessed to determine the effects of oleocanthal, based on oxidative stress and serum mediators of inflammation by enzyme-linked immunosorbent assay (ELISA). Quantitative Polymerase Chain Reaction (qRT-PCR) was used to estimate the mRNA expression of Toll-like receptor 4 (TLR4) and Interleukin 1 Receptor Associated Kinase 4 (IRAK4) proteins in the cerebral tissue of rats CPB-induced injury. Western blot assay and histopathology were also performed. Results:The findings suggest that pre-treatment with oleocanthal reduced neurological dysfunction and cerebral injury. Parameters of oxidative stress and cytokine levels were reduced in the serum of the oleocanthal treated group compared with the CPB-only group. Pre-treatment with oleocanthal ameliorated the expression of TLR-4, IRAK4, and Zonula occludens-1 (ZO-1) proteins in the cerebral tissue of the CPB-injured rats. Conclusions:The results revealed that treatment with oleocanthal protected against cerebral damage by controlling microglia inflammation through the TLR-4 pathway.

中文翻译：

---

Oleocanthal 通过调节 NLRP3 通路保护大鼠免受神经元炎症和体外循环手术引起的脑损伤

背景：心脏直视手术是在可能导致神经元损伤的体外循环 (CPB) 技术的帮助下进行的。目的：本研究调查了油橄榄预处理对 CPB 诱导的脑损伤的潜在保护作用。方法：治疗组于CPB诱导前3h腹腔注射油橄榄油30mg/kg。基于氧化应激和炎症的血清介质，通过酶联免疫吸附试验 (ELISA) 评估了行为神经学评分和脑损伤，以确定油橄榄的作用。定量聚合酶链反应 (qRT-PCR) 用于估计大鼠 CPB 损伤脑组织中 Toll 样受体 4 (TLR4) 和白细胞介素 1 受体相关激酶 4 (IRAK4) 蛋白的 mRNA 表达。还进行了蛋白质印迹测定和组织病理学。结果：研究结果表明，油橄榄预治疗可减少神经功能障碍和脑损伤。与仅使用 CPB 的组相比，油橄榄处理组的血清中氧化应激参数和细胞因子水平降低。油橄榄预治疗改善了 CPB 损伤大鼠脑组织中 TLR-4、IRAK4 和封闭带蛋白-1 (ZO-1) 蛋白的表达。结论：结果表明，油橄榄素治疗可通过 TLR-4 通路控制小胶质细胞炎症，从而防止脑损伤。与仅使用 CPB 的组相比，油橄榄处理组的血清中氧化应激参数和细胞因子水平降低。油橄榄预治疗改善了 CPB 损伤大鼠脑组织中 TLR-4、IRAK4 和封闭带蛋白-1 (ZO-1) 蛋白的表达。结论：结果表明，油橄榄素治疗可通过 TLR-4 通路控制小胶质细胞炎症，从而防止脑损伤。与仅使用 CPB 的组相比，油橄榄处理组的血清中氧化应激参数和细胞因子水平降低。油橄榄预治疗改善了 CPB 损伤大鼠脑组织中 TLR-4、IRAK4 和封闭带蛋白-1 (ZO-1) 蛋白的表达。结论：结果表明，油橄榄素治疗可通过 TLR-4 通路控制小胶质细胞炎症，从而防止脑损伤。