Subcellular organelles play indispensable roles in diverse biological processes by their precise mutual cooperation. Thus, the development of a single fluorescent probe (SF-probe) for simultaneous and discriminable visualization of different organelles and their dynamics during certain bioprocess is significant, yet remains greatly challenging. Herein, for the first time, we rationally prepared a pH-sensitive SF-probe (named HMBI) for the simultaneous two-color visualization of nuclei and mitochondria and monitoring cell apoptosis. HMBI shows remarkable ratiometric fluorescence changes toward pH changes. Due to different pH environments in subcellular organelles, HMBI can image nuclei and mitochondria with green and red emission, respectively. HMBI can monitor drug-induced cell apoptosis with dramatically decreased red emission in mitochondria but almost unchanged green emission in nuclei, and the shrinking and pyknotic nuclei are also observed during cell apoptosis. HMBI possesses tremendous potential in two-color biomedical imaging of the dynamic changes of nuclei and mitochondria in many physiological processes.

中文翻译：

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单个荧光pH探针可同时进行核和线粒体的两种颜色可视化并监测细胞凋亡

亚细胞细胞器通过精确的相互合作在多种生物过程中起着不可或缺的作用。因此，开发一种荧光探针（SF-probe）用于同时可视化不同细胞器及其在某些生物过程中的动力学的过程是很重要的，但仍然具有很大的挑战性。本文中，我们首次合理地制备了pH敏感的SF探针（名为HMBI），用于同时进行核和线粒体的双色可视化并监视细胞凋亡。HMBI显示出显着的比例荧光变化，朝向pH值变化。由于亚细胞器中的pH值环境不同，HMBI可以分别以绿色和红色发射来成像细胞核和线粒体。HMBI可以监测药物诱导的细胞凋亡，线粒体中的红色发射显着减少，而细胞核中的绿色发射几乎没有变化，并且在细胞凋亡过程中也观察到了收缩的和致密的细胞核。HMBI在双色生物医学成像中具有许多生理过程中核和线粒体动态变化的巨大潜力。