Abstract

Silymarin is used for a wide variety of biological applications including, antidiabetic activities. However, the effectiveness of Silymarin is affected by its poor aqueous solubility and low systemic bioavailability after oral administration. The present study aimed to formulate a new, simple, and inexpensive form of silymarin solution. A new form of silymarin solution (NFSM) characterised by small particle size (227.5 nm), high entrapment efficiency (>82%), and appropriate zeta potential(–24.7mv). Moreover, the antidiabetic effects of NESM were evaluated relative to native Silymarin (SM). Oral administration of NFSM for 14 days in diabetic rats significantly decreased fasting blood glucose, oxidative stress levels, and improved lipid profile compared with SM. Also, NFSM significantly increased serum insulin levels, the gene expression of insulin and Pdx1, restored and improved the structure of the liver, and pancreas histologically. Our results concluded that NFSM may be an efficient carrier for oral delivery of silymarin for the management of diabetes and aggravated antioxidant status.

摘要

水飞蓟素被用于多种生物应用，包括抗糖尿病活性。然而，水飞蓟素的有效性受其水溶性差和口服给药后全身生物利用度低的影响。本研究旨在制定一种新型，简单且廉价的水飞蓟素溶液形式。一种新型的水飞蓟素溶液（NFSM），其特征在于小粒径（227.5 nm），高包封率（> 82％）和适当的Zeta电位（–24.7mv）。此外，相对于天然水飞蓟素（SM）评估了NESM的抗糖尿病作用。与SM相比，在糖尿病大鼠中口服NFSM 14天可显着降低空腹血糖，氧化应激水平并改善脂质状况。此外，NFSM显着增加了血清胰岛素水平，胰岛素和Pdx1的基因表达在组织学上恢复并改善了肝脏和胰腺的结构。我们的结果得出结论，NFSM可能是水飞蓟素口服给药的有效载体，用于治疗糖尿病和加剧抗氧化剂状况。