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Prevalence and molecular characterization of Turkish isolates of the rose viruses
Crop Protection ( IF 2.5 ) Pub Date : 2021-02-03 , DOI: 10.1016/j.cropro.2021.105565
Ali Karanfil

Roses are among the most important ornamental plants in the world. There are many biotic disease factors that cause damage to this important plant. The most important group among these is virus diseases. However, of the studies about rose viruses conducted worldwide, those in Turkey are limited. With the aim of determining the prevalence and genetic diversity of rose viruses, 332 rose plants showing virus and virus-like symptoms were collected in 14 provinces from four different geographic regions of Turkey. The samples were tested to determine the infection of nine different viruses infecting rose plants. As a result of the tests, prunus necrotic ringspot (PNRSV), rose cryptic virus-1 (RCV-1), rose spring dwarf-associated virus (RSDaV), rose yellow vein virus (RYVV), and apple mosaic virus (ApMV) infections were detected. It was determined that 94.27% of the samples were infected with at least one virus infection. While the virus causing the most infection was found to be PNRSV, the least one was determined as ApMV. With this result, PNRSV is determined as a significant virus disease for rose plants in Turkey. Based on their geographical origins, 25 PNRSV and RCV-1 isolates, 15 RSDaV and RYVV isolates, and 10 ApMV isolates were selected for further analysis and their partial genetic diversity was investigated. As a result of these analyzes, it was determined that PNRSV, RCV-1, and RSDaV isolates showed a high-sequence homology with each other and with world isolates. Rose ApMV isolates were found to form separate groups phylogenetically. RYVV, which is known to exist in a very limited number of countries, has been found to have up to 10% genetic differences and phylogenetically, three different groups were determined. Furthermore, to the best of our knowledge, these are the first reports of RCV-1 and RSDaV in Turkey. It was also found that Turkey is still free of rose rosette virus. Therefore, quarantine measurements should continue to be strictly enforced.



中文翻译:

玫瑰病毒土耳其分离株的流行和分子特征

玫瑰是世界上最重要的观赏植物之一。有许多生物病害因素导致对该重要植物的损害。其中最重要的人群是病毒疾病。但是,在全世界进行的有关玫瑰病毒的研究中,土耳其的研究有限。为了确定玫瑰病毒的流行性和遗传多样性,从土耳其四个不同地理区域的14个省中收集了332株显示病毒和类病毒症状的玫瑰植物。测试样品以确定感染玫瑰植物的九种不同病毒的感染。测试的结果是:李属坏死环斑(PNRSV),玫瑰隐病毒1(RCV-1),玫瑰春矮伴病毒(RSDaV),玫瑰黄脉病毒(RYVV)和苹果花叶病毒(ApMV)检测到感染。已确定至少有一种病毒感染了94.27%的样品。虽然发现造成感染最多的病毒是PNRSV,但确定最少的病毒是ApMV。结果,PNRSV被确定为土耳其玫瑰植物的重要病毒病。根据它们的地理起源,选择了25个PNRSV和RCV-1分离株,15​​个RSDaV和RYVV分离株以及10个ApMV分离株进行进一步分析,并研究了它们的部分遗传多样性。这些分析的结果是,确定PNRSV,RCV-1和RSDaV分离株彼此之间以及与世界分离株之间显示出高序列同源性。发现玫瑰ApMV分离株在系统发育上形成单独的基团。RYVV,在少数国家/地区存在,已经发现具有高达10%的遗传差异,在系统发育上,确定了三个不同的组。此外,据我们所知,这是土耳其RCV-1和RSDaV的首次报道。还发现土耳其仍然不含玫瑰花结病毒。因此,应继续严格执行检疫措施。

更新日期:2021-02-09
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